Protein Info for RALBFv3_RS19795 in Ralstonia solanacearum IBSBF1503
Annotation: multidrug efflux RND transporter permease subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 65% identity to abo:ABO_0964)MetaCyc: 59% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1051 amino acids)
>RALBFv3_RS19795 multidrug efflux RND transporter permease subunit (Ralstonia solanacearum IBSBF1503) MARYFIDRPIFAWVLAIIVMLAGALSIATLPIAQYPSIAPPAVAITATYPGASAKTLEDT VTQIIEQKMKGLDHLSYMASTSESSGSVTITLTFENGTDPDTAQVQVQNKLALATPLLPQ EVQQQGVQVTKSATNFLNVLAFTSEDGSMNGSDLSDYVAANVQDAISRVEGVGDTTLFGS QYAMRIWLDPNKLSNFSLTPLDVKTAIQAQNAQVSAGQLGAMPALGNQQINATITSQTRL KTVEEFGNILLRTQTDGAQVRLRDVARIELGSENYNTVGRYNGKPAAGLAIKLATGANAL DTVKAIDARVAELEPFFPPGMRVQKPYDTTPFVRISIEEVVRTLVEAVALVFLVMYLFLQ NFRATLIPTIAVPVVLLGTFGVLAACGFTINTLTMFAMVLAIGLLVDDAIVVVENVERVM SEEGLPPKEATRKSMGQITGALVGVALVLAAVFVPMAFFKGSTGVIYRQFSITIVSAMTL SVLVALVLTPALCATLLKPVKKGHAQATTGFFGWFNRTFDRGNHRYQGIVRHMLGKSRRY MAVYGVLFAMVVVGFMKLPVGFLPDEDQGTLFVLAQLPPGATNARTEDVIRQVEHHFLVD QKEAVSGVFSVAGFSFAGSGQNMGFAFVKLRPWNERKGEALSVNGVVAKAFGFFATLRDA RVFAFAPPAVAELGNATGFDLMLQDRANLGHAALMQARNQLLGTLSQDQRLVAVRPNGQE DTPEFQLQIDTHKAETLGVSISDINNTLATAWGSSYVNDFIDRGRVKKVMLQADAPFRMN PGDIDKWYVRNSAGTMVPFTAFAKVDWASGSPRLERYNGVPSMEILGMAKPGSASSGEAL AIVEDAVAKLPAGIGYSWTGLSLQEKASTGQTTLLYTLSILFVFLCLAALYESWAIPFSV IMVVPLGVLGALIGAILTWKMNDVYFQVGLLTTIGLASKNAILIVEFAKDLHAQGMRLAE AAMTAARMRLRPILMTSLAFILGVLPMVTGSGAGAGAQHALGTAVIGGMLSGTVLAIFFV PLFFVLVRGLFRRKPATVAVTAAQPADAATS