Protein Info for RALBFv3_RS19700 in Ralstonia solanacearum IBSBF1503

Annotation: nicotinate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF18127: NAMPT_N" amino acids 16 to 107 (92 residues), 110 bits, see alignment E=5.8e-36 PF04095: NAPRTase" amino acids 179 to 420 (242 residues), 190.8 bits, see alignment E=3.3e-60

Best Hits

Swiss-Prot: 46% identical to NAMPT_MOUSE: Nicotinamide phosphoribosyltransferase (Nampt) from Mus musculus

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 95% identity to rso:RS05359)

MetaCyc: 64% identical to nicotinamide phosphoribosyltransferase (Synechocystis sp. PCC 6803)
Nicotinamide phosphoribosyltransferase. [EC: 2.4.2.12]

Predicted SEED Role

"Nicotinamide phosphoribosyltransferase (EC 2.4.2.12)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 2.4.2.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.11

Use Curated BLAST to search for 2.4.2.11 or 2.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>RALBFv3_RS19700 nicotinate phosphoribosyltransferase (Ralstonia solanacearum IBSBF1503)
MQNDLPGLSAILSNPILNTDSYKASHYLQYPAGTSAMFSYVESRGGRYDRTVFFGLQMLL
KEYLCRPITPAMIDAARGFFAAHGEPFNEAGWRYIVERYDGYLPVRIRAVPEGSVVPTHN
VLMTIECDDPEVFWLASYLETMLLRVWYPITVATQSWHLRQLVRRYLEQTSDDPGQLPFK
VHDFGARGVSSAESSAIGGAAHLVSFMGSDTVLGVVAANTYYNAQMAAFSVPAAEHSTIT
AWGRAGEADAYRNMLRQFGQPGALVSVVSDSYDLFAALRLWGGELRQAVIDSGATLVVRP
DSGDPRSIVLQTVRALDASFGAAVNGKGYRVLNHVRVIQGDGINAGSIEAILSALEAAGY
AADNVVFGMGGALLQKLNRDTQRFAMKCSAVRVGGAWRDVCKDPVTDAGKRSKKGRLTLL
RNRVSGEYATATLPLAWDDRRVEGDWEDALVTVFENGRLLRDVSLEAVRALAQAHEAVPE
LVD