Protein Info for RALBFv3_RS19440 in Ralstonia solanacearum IBSBF1503

Annotation: polygalacturonase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00295: Glyco_hydro_28" amino acids 92 to 461 (370 residues), 155.3 bits, see alignment E=1.1e-49

Best Hits

Swiss-Prot: 94% identical to PGLR_RALSL: Polygalacturonase (pglA) from Ralstonia solanacearum

KEGG orthology group: None (inferred from 89% identity to rsl:RPSI07_mp0827)

Predicted SEED Role

"macromolecule metabolism; macromolecule degradation; degradation of polysaccharides"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>RALBFv3_RS19440 polygalacturonase (Ralstonia solanacearum IBSBF1503)
MNHRYTLLALAAAALSAGAHATGTSVTAPWGKVAEPSLPADSAVCKTLSASITPIKDSVD
SVDGNPANSQPDASRIQSAIDNCAAGQAVKLVKGSAGESGFLSGSLKLKSGVTLWIDSGV
TLFASRNPADYDNGLGTCGTATTSNDKSCNALIVARDTAGSGIVGAGAIDGRGGSLVTSG
PNANQLTWWDIAYLNKTKGLHQQNPRLIQTYNGSAFTLYGVTVQNSPNFHIVTTGTSGVT
AWGIKIVTPSLAYAVAGYKCPTGTTPDKVTPATCFTPETVKNTDGFDPGQSTNVVLAYSY
ISTGDDHVAIKASSGATRNLLFAHNHFYYGHGLSIGSETNGGVSNMLVTDLTMDGNNSSD
DNDRSAGNGLRIKSDASRGGKVNNIVYDGICMRNVQVPLVFDPFYSSSKGTLYPNFTNIV
VRNFHDLGSAKLTKRTMTFLGYEANKQKNPLKITLDNVVFDGTLPAFDGAHNGGPASPNG
VHFTFGGTGPVSFADAIVTSSTTDVTVTGTPGTAAAVDCSKAFVPLKSVVPTSPI