Protein Info for RALBFv3_RS18785 in Ralstonia solanacearum IBSBF1503

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 74 to 381 (308 residues), 143.9 bits, see alignment E=2.9e-46 PF16576: HlyD_D23" amino acids 86 to 304 (219 residues), 199.5 bits, see alignment E=6.5e-63 PF13437: HlyD_3" amino acids 202 to 301 (100 residues), 70 bits, see alignment E=3.9e-23

Best Hits

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 92% identity to rsl:RPSI07_mp1076)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (388 amino acids)

>RALBFv3_RS18785 efflux RND transporter periplasmic adaptor subunit (Ralstonia solanacearum IBSBF1503)
MKKFSPLVALIALAAAVAVGVVVGRNWPSAAHGEADKTAAATPGKTDYAAAASQLRAPVD
TVAIPDGGFDPQQVQVAQVTQQNLPVELDTPGKLAYNAERTKLASARVAGRLDRILVFEG
ALVHEGQPLAELYAPDYISAQKEYLLALNTARTLQGSTMKDLQDDAQQTAQSAAGRLHVL
GLTDAEIAQIRARGTPAEHLILRAPISGTIVKRNMDPGAFLNVGDSFMSIVDTRALWFTG
NVYENDIARVRIGQPITLRTAAYPDRAFTGTVSFIAPNIDAATHTLTVRCDVPNPDGLLR
PEMYSTARIQTGQAMGTVVPKTALVKDHGRYYVIVQSDAGHFRRTEVQGHDLPGADAGFA
VTGGLSPQSAVVVRGAALLNEMIVKAGA