Protein Info for RALBFv3_RS18670 in Ralstonia solanacearum IBSBF1503

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 PF00358: PTS_EIIA_1" amino acids 9 to 129 (121 residues), 133.3 bits, see alignment E=1e-42 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 10 to 129 (120 residues), 119.6 bits, see alignment E=1.2e-38 PF00381: PTS-HPr" amino acids 180 to 256 (77 residues), 77.1 bits, see alignment E=2.3e-25 TIGR01003: phosphocarrier, HPr family" amino acids 181 to 255 (75 residues), 61.9 bits, see alignment E=6.7e-21 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 295 to 844 (550 residues), 489.4 bits, see alignment E=2e-150 PF05524: PEP-utilisers_N" amino acids 296 to 413 (118 residues), 72.7 bits, see alignment E=7.4e-24 PF00391: PEP-utilizers" amino acids 438 to 511 (74 residues), 81.1 bits, see alignment E=9.9e-27 PF02896: PEP-utilizers_C" amino acids 540 to 821 (282 residues), 345.6 bits, see alignment E=5.5e-107

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 90% identity to rsl:RPSI07_mp1099)

Predicted SEED Role

"PTS system, glucose-specific IIA component / Phosphocarrier protein of PTS system / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Mannitol Utilization or Fructose utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (850 amino acids)

>RALBFv3_RS18670 phosphoenolpyruvate--protein phosphotransferase (Ralstonia solanacearum IBSBF1503)
MSSRHLQLRVLAPLSGRVVPIEAVPDPAFAQKMVGDGLSIDPATDLVLAPIDGRVIDFHE
ARHALVIAHACGVEIMVHVGLDTVLLAGQGFEALVGKGDTVRAGQPLLRFDAAHVAAKAS
TLTEIVVVNGGERVRQMDKAAGSVEAGLSELLILHLEAALEASGEPAQAEEDALLHSPPV
VLPNPAGLHARPAARLASEARAFAARITLWCGQQQADAKSAVAVMALATRCGDEVRLSGC
GNDAAQALAVLAELLRNGCGESAGAATAPSAQAAAPIPQNRPAVLAPAQAGGLLCGVGAS
PGLAVGRIVRWSGIAPTFDETGGPFEQEHARLHAALQIAASQIKSLALADTLPDAPQAQI
MDAHLTFLEDPMLVEAAETHLRSGASAESAWHLACEAVEQGLRQHANAQVRERAADVRDV
GARVLTVLTGATPRPRSLPERSIIIADDLTPSDTIAMDRSKVAGLCTVSGGPTSHVAILA
RSMGIPAVCGVPAQALSLQDGTLAVLDGTAGVLQADPDPTLRAEVERRQAAAHQQQQVDQ
QTAHRPGRTRDGHRVEVVANVRDAAEAREAVAAGGEGVGLLRSEFLFEDRAAPPDEDEQA
AAYQAVAAALGPERPLIARTLDIGGDKPLRYLPLPKEENPFLGLRGIRVSLAYPELFRSQ
LRAMLRAASGGNLHIMFPMVSDLDEVLAAKRILREEQARYPAPVKIGLMIEVPAAVAIVE
ALAQEVDFFSIGTNDLTQYTLAIDRGHAGLASKVDALHPAVLRMIALTVEGAHAHGKWVG
VCGALASDLAAVPVLVGLGVDELSVSVPAIAAVKAALSRLDFEDCRALARRVLPLGSASR
VREVLAQRLV