Protein Info for RALBFv3_RS18330 in Ralstonia solanacearum IBSBF1503

Annotation: amino acid ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00497: SBP_bac_3" amino acids 36 to 266 (231 residues), 97 bits, see alignment E=1.2e-31 PF12974: Phosphonate-bd" amino acids 67 to 262 (196 residues), 43.1 bits, see alignment E=5.3e-15 PF09084: NMT1" amino acids 79 to 246 (168 residues), 26.6 bits, see alignment E=7.9e-10

Best Hits

Swiss-Prot: 43% identical to GLTI_SALTY: Glutamate/aspartate import solute-binding protein (gltI) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K10001, glutamate/aspartate transport system substrate-binding protein (inferred from 93% identity to rsl:RPSI07_mp1173)

MetaCyc: 41% identical to glutamate/aspartate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-13-RXN [EC: 7.4.2.1]; 7.4.2.1 [EC: 7.4.2.1]

Predicted SEED Role

"Glutamate Aspartate periplasmic binding protein precursor GltI (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>RALBFv3_RS18330 amino acid ABC transporter substrate-binding protein (Ralstonia solanacearum IBSBF1503)
MLPRVRKLLPALLVFPLMNAVASSPTLDRIKATGAIRLAHRESSVPFSYLDAGGKPIGYS
MDLCMRLVDAIRENLKRPDLKVQYLQVTPATRMDAIMNGKADLECASTNNARERREKVAF
TIPHYIANGRMLVKTASGIQKLEDLRGKTVVSTRGSANGDLVRQLSEAGGLGITVVEASD
HAQSFAMLADGKAQAFAMDDVLLAGMKATTKNPADYAIVGKTQQVVPYAIMLSKNDPEFK
KLIDAAMSRLMEDGEAERFYKKWFQQPIPPNNVNLGIPMSMLLRDSFRYPTDNAGN