Protein Info for RALBFv3_RS18010 in Ralstonia solanacearum IBSBF1503

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 915 PF04879: Molybdop_Fe4S4" amino acids 14 to 64 (51 residues), 62.2 bits, see alignment 7.1e-21 PF00384: Molybdopterin" amino acids 69 to 507 (439 residues), 251.5 bits, see alignment E=2.6e-78 PF01568: Molydop_binding" amino acids 609 to 720 (112 residues), 102 bits, see alignment E=3.9e-33 PF04324: Fer2_BFD" amino acids 858 to 907 (50 residues), 47.6 bits, see alignment 3.4e-16

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 96% identity to rsl:RPSI07_mp1245)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (915 amino acids)

>RALBFv3_RS18010 nitrate reductase (Ralstonia solanacearum IBSBF1503)
MTMTTIPLHPLTEAVRTTCPYCGVGCGVLATPRADGSVGIAGDPAHPANAGRLCVKGSAL
GETVDLDGRLLHPAMRARTVDGDGDASPAPLRRVSWDAALDAVAQGFRRIVDTHGPDAVA
LYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYDDLE
AADLIVLVGSNTAWCHPILFQRIVRVKEARPEVKVVVIDPRHTATCELADLHLPIKPGTD
VWLFNGLLSFLARRGAVDRNFVDLHTGGLDEALRTADAEADDVAAVARACKLKLDDLLTF
YGWFADTQRTITAFSQGVNQSSAGTDKVNSIINCHLLTGRIGRAGMGPFSITGQPNAMGG
REVGGLANMLAAHLELHEPRHRELVQTFWNAPRMADKVGLKAVDLFEAIEAGRVKAVWIM
GTNPVVSLPDADQVRRALAKCELVVASDIVEATDTTLLADIVLPALGWGEKDGTVTNSER
RISRQRAFLPAPGEARADWDIIADVARRMGYDGFDFKSGHEVFDEHARLSAFANDAQGVR
RAFHLGGLTGLDAGAYDGMAPVQWPVTLSGASEARLFSDGRFAHADGRARFVPTPARAPV
HAPDSDYPLILNTGRVRDQWHTMTRTGRAPKLADHVPEPFVDLHPHDALLAGVREGALAR
VGSRWGSVVARVRMSGGIARGSVFVPIHWNGQFASDARVGAVVNPVVDPVSGEPEFKHTP
VMVEPFDVAWHGFVLSRRALDTDEATWWTRIQGRRFVRYELAGRQPIGSAHAWARRLLGV
DDPHADWLEYEDTTARVYRAVHVVDDRIEACVFLSPRPDLPSRNWLAGLFAHDRLEDIDR
AGVLLGQPIEAGADAGPTVCSCFGVGRNTICDAIRTKGLQSTGEITACLKAGGNCGSCVP
ELKRLLVEARVQQAA