Protein Info for RALBFv3_RS17700 in Ralstonia solanacearum IBSBF1503

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 711 PF00580: UvrD-helicase" amino acids 21 to 311 (291 residues), 205.5 bits, see alignment E=2.6e-64 PF13245: AAA_19" amino acids 37 to 297 (261 residues), 73.7 bits, see alignment E=3.4e-24 PF13361: UvrD_C" amino acids 319 to 472 (154 residues), 80.1 bits, see alignment E=4.4e-26 amino acids 579 to 641 (63 residues), 62.7 bits, see alignment E=8.6e-21 PF13538: UvrD_C_2" amino acids 585 to 639 (55 residues), 41 bits, see alignment 2.7e-14

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 95% identity to rsl:RPSI07_mp1314)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (711 amino acids)

>RALBFv3_RS17700 ATP-dependent helicase (Ralstonia solanacearum IBSBF1503)
MPAAAPTPQPPLTPDYLAQLNDQQRQAVEFGVSGADAAETGPLLVLAGAGSGKTQTLGWR
VAHLVAHGADPQRILLLTFSRRAASELSSRAGHLLARAMQGNTAGTRSAAGTTYQTALPW
AGTFHGIGARLLREYAERIGLASDFTIHDRSDSADLLNVVRHELNLSTKARRFPLKQTCL
AIYSRAINAESPLDAVLKQHFPWCAMWQAQLRTLFDAYVQAKQAQHVLDYDDLLLYWHAM
VSDAGLAAEIGARFDHILVDEYQDTNRLQATILRALRPDGRGLTVVGDDAQSIYAFRAAT
VRNILDFPRHFTRPARTVLLERNYRSTQPILDASNGVMRFATERFAKTLWTDRASTHRPR
LVSVRDEAEQARCVAEQVLQHREGGLALKSQAVLFRTASHSAPLEIELTRRNIPFVKYGG
LKFLEASHVKDVLSVLRWAENPRNRIAGFRVIQLLPGAGPATAARVLDAMAEAADPIAAL
LAHEPPARLAGDWAALMTLMATLRMPKDDAAWAGELGAVGAWYAPHLERLHDDAAVRQGD
LDQLARMAATYASRERFLTEMTLDPPELTSDESGAPYRDEDYLILSTIHSSKGQEWKAVY
LLNAVDGCMPADLGAGSHDELEEERRLLYVAMTRARDHLDILVPQRFYVTQQTPNGDRHI
YASRTRFIPPALLPLFEACAWPPPEPGADPAAEARAAAKVDLAKKMRGYWR