Protein Info for RALBFv3_RS16750 in Ralstonia solanacearum IBSBF1503

Annotation: nitrate reductase molybdenum cofactor assembly chaperone

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR00684: nitrate reductase molybdenum cofactor assembly chaperone" amino acids 14 to 165 (152 residues), 99.9 bits, see alignment E=7e-33 PF02613: Nitrate_red_del" amino acids 37 to 162 (126 residues), 48.2 bits, see alignment E=5.9e-17

Best Hits

KEGG orthology group: K00373, nitrate reductase 1, delta subunit [EC: 1.7.99.4] (inferred from 91% identity to rsl:RPSI07_mp1506)

Predicted SEED Role

"Respiratory nitrate reductase delta chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (222 amino acids)

>RALBFv3_RS16750 nitrate reductase molybdenum cofactor assembly chaperone (Ralstonia solanacearum IBSBF1503)
MMTNAMLFRLLAAMLDYPQAELIEALPEIESRLAARPDARARLVPLLDLLRGSDLIALEE
NYVATFDRNPSHALYLFEHIHGESRDRGQAMVDLVDEYRKYGYEPASSELPDFVPLFLET
LGMMAAAGHADDATALLGDAIHVLAAIGERLARNGSPYATVFDVLRTLSDVQPQPQEDPP
VRDMEEAIERFGPGADGVEPLLTPRPPEVQPVHFHPRAPQPH