Protein Info for RALBFv3_RS15700 in Ralstonia solanacearum IBSBF1503

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details PF16576: HlyD_D23" amino acids 70 to 300 (231 residues), 47.4 bits, see alignment E=3.8e-16 PF13533: Biotin_lipoyl_2" amino acids 72 to 120 (49 residues), 30.5 bits, see alignment 6e-11 PF00529: CusB_dom_1" amino acids 182 to 357 (176 residues), 57.8 bits, see alignment E=2.2e-19 PF13437: HlyD_3" amino acids 231 to 349 (119 residues), 50.3 bits, see alignment E=8.7e-17

Best Hits

Swiss-Prot: 47% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 98% identity to rsc:RCFBP_20134)

MetaCyc: 47% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>RALBFv3_RS15700 hemolysin D (Ralstonia solanacearum IBSBF1503)
MSDNTPQAAPSAAPAPAASAATPTGNGNGKRKRMLTTLATALVVAGIGYGLYWGLYGRWF
ESTDDAYVQGNVVQVTPQVAGTVVAIRADDTQLVTSGQPLIELDRADARVALEQSEAALA
QAVRQVRTLYSNTGAYTSTLAMRESDLAKAKEDLTRRKQIAGTGAVSQEEIAHAQISLQA
AEAAVETAKEQLQANRVLTDQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAKR
SVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGGSVTYHGKV
VGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEARVDIHD
EGGQSLASTSATSPLQTSVYDQAGQDADQVIASIIATNAGRGAASAPAAGSASAPASAKR
AAQPAPKA