Protein Info for RALBFv3_RS14800 in Ralstonia solanacearum IBSBF1503

Annotation: 3-deoxy-8-phosphooctulonate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 97% identical to KDSA_RALPJ: 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 100% identity to rsc:RCFBP_20307)

MetaCyc: 49% identical to 3-deoxy-8-phosphooctulonate synthase subunit (Arabidopsis thaliana col)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
CMP-3-deoxy-D-manno-octulosonate biosynthesis (5/5 steps found)
CMP-8-amino-3,8-dideoxy-D-manno-octulosonate biosynthesis (4/7 steps found)
superpathway of Kdo₂-lipid A biosynthesis (14/25 steps found)

KEGG Metabolic Maps

Lipopolysaccharide biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (284 amino acids)

>RALBFv3_RS14800 3-deoxy-8-phosphooctulonate synthase (Ralstonia solanacearum IBSBF1503)
MKLCDFEIGLDRPFFLIAGTCVIESEQMAIDTAGTLKEITGALGIPFIYKSSFDKANRSS
DTSFRGLGMEAGLRILGEVRRQVGVPVLTDVHEIDEIKPVAEVVDVLQTPAFLCRQTDFI
RACAQSGKPVNIKKGQFLAPHDMKNVIDKARHAARDAGLPEDSFMACERGASFGYNNLVS
DMRSLAIMRETGAPVVFDATHSVQLPGGQGTSSGGQREFVPVLARAAIATGVAGVFMETH
PDPACAKSDGPNAVPLRRMKDLLSVLKELDALTKRSGFLENQFD