Protein Info for RALBFv3_RS14620 in Ralstonia solanacearum IBSBF1503

Annotation: gentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 TIGR02272: gentisate 1,2-dioxygenase" amino acids 9 to 347 (339 residues), 606.9 bits, see alignment E=4.4e-187 PF07883: Cupin_2" amino acids 93 to 163 (71 residues), 63.6 bits, see alignment E=1.7e-21

Best Hits

KEGG orthology group: K00450, gentisate 1,2-dioxygenase [EC: 1.13.11.4] (inferred from 96% identity to rsl:RPSI07_2304)

MetaCyc: 54% identical to gentisate 1,2-dioxygenase I monomer (Pseudomonas alcaligenes NCIMB 9867)
Gentisate 1,2-dioxygenase. [EC: 1.13.11.4]; 1.13.11.4 [EC: 1.13.11.4]; 1.13.11.4 [EC: 1.13.11.4]

Predicted SEED Role

"Gentisate 1,2-dioxygenase (EC 1.13.11.4)" in subsystem Gentisare degradation or Salicylate and gentisate catabolism (EC 1.13.11.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (348 amino acids)

>RALBFv3_RS14620 gentisate 1,2-dioxygenase (Ralstonia solanacearum IBSBF1503)
MTSQTLPLERGTYYSRLGGMGLAPLWESLHALVPREPKPRIVPARWQYEAIRPLVMQAGS
VISAEEAVRRVLVLENPGLPGQSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGEG
AWTAVNGERTTMHPGDFIITPSWTWHDHGCPRIDEGGEPVVWLDGLDIPLVQQFDAGFAE
NYPEAQQPVTRPEGDSFARFGHNMVPVRHRVTDPTSPIFSYPYERSREALDQLYRHGELD
AWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFQGKTYRSTDATVYCVVEGHGTAHIGDA
RFAFAPHDVFVAPSWAPVQLDARDNAVLFSYSDRPVLAALNLLREART