Protein Info for RALBFv3_RS13870 in Ralstonia solanacearum IBSBF1503

Annotation: D-amino acid dehydrogenase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF00070: Pyr_redox" amino acids 2 to 42 (41 residues), 28.7 bits, see alignment 6.2e-10 PF01266: DAO" amino acids 2 to 397 (396 residues), 279.7 bits, see alignment E=1.9e-86 PF00890: FAD_binding_2" amino acids 3 to 258 (256 residues), 26.3 bits, see alignment E=1.8e-09 PF02558: ApbA" amino acids 3 to 33 (31 residues), 23.2 bits, see alignment (E = 1.9e-08) PF13450: NAD_binding_8" amino acids 5 to 42 (38 residues), 25.4 bits, see alignment 5.7e-09 PF01593: Amino_oxidase" amino acids 205 to 255 (51 residues), 22.4 bits, see alignment 2.8e-08

Best Hits

Swiss-Prot: 97% identical to DADA1_RALSO: D-amino acid dehydrogenase 1 (dadA1) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 99% identity to rsc:RCFBP_20555)

MetaCyc: 68% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>RALBFv3_RS13870 D-amino acid dehydrogenase small subunit (Ralstonia solanacearum IBSBF1503)
MRVLVLGSGVIGVTSAYYLARAGHEVTVVDREAGPALDTSFANAGQISPGYASPWAAPGV
PLKAIKWMFQQHAPLSIRPDGTLFQLQWMWQMLRNCNAASYAENKERMVRLAEYSRDCIR
ALRAETGIAYEGRQQGTLQVFRTQQQLDGAANDIAVLERAGVPYELLSRDDLVRSEPGLA
ATRHKLAGGLRLPNDETGDCQLFTTRLAAMAEQLGVRFRFNSRINSLIVQNDAVRGALVD
GEAMTADLVVVAMGSYSTPFLKGLVGVPVYPLKGFSITVPMTDTEHSPVSTVLDETYKVA
ITRFDDRIRVGGMAQIVGYDKRLDPGKRKTLEFVVNDLFPGGGDVSRATFWTGLRPMTPD
GTPIVGPTPVRGLWLNTGHGTLGWTMACGSGKLLSDLVSGRSPAIRADDLSVYRYLHGGQ
VPATKPAIA