Protein Info for RALBFv3_RS11700 in Ralstonia solanacearum IBSBF1503

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 14 to 404 (391 residues), 216 bits, E=1.2e-67
• PF13450: NAD_binding_8
  • amino acids 16 to 43 (28 residues), 25.6 bits, see alignment (E = 1.2e-09)

Best Hits

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 97% identity to rsc:RCFBP_20974)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

• Nitrogen metabolism
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Protein Sequence (423 amino acids)

>RALBFv3_RS11700 FAD-dependent oxidoreductase (Ralstonia solanacearum IBSBF1503)
MNHNAADTAGRRTITVVGAGIVGVSCALQLQRDGHRVTLLDQAGIGEGCSYGNAGCLSVA
SVVAMALPGMLAQVPKWLADPQGPLTVRWSYLPRALPWLLKWIRAGTEAQARAVARPLAD
LFSATYPGYRSLLEPAQYDDLIRATGHLYVWRTLARSSGEVLAQSIRDATGVRSQALSAG
EVRDLEPALAGDIQSGLLLPDNGFTCNPERLVKTLAARFVAAGGTVLRRKVLDFTLGERG
PTRLYTDCGSLPVSTLVICAGAWSMRLGARLTGVRVPLDTERGYHAMLRAPTVQPSRPIM
DCERKFIATPMEDGLRIAGTVEIGGLDIPPDFRRAGTLTRHGQALFPGLAFADDSAWMGY
RPSLPDSLPVIDRSERFADVFFAFGHGHLGMTGAPGTGRLIADLVAGRPPFIDARPYGLQ
RFA