Protein Info for RALBFv3_RS11590 in Ralstonia solanacearum IBSBF1503

Annotation: dihydroorotase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00856: dihydroorotase, homodimeric type
  • amino acids 5 to 342 (338 residues), 478 bits, E=7.4e-148
• PF01979: Amidohydro_1
  • amino acids 12 to 311 (300 residues), 75.5 bits, E=2.4e-25

Best Hits

Swiss-Prot: 98% identical to PYRC_RALSO: Dihydroorotase (pyrC) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 98% identity to rso:RSc0487)

MetaCyc: 57% identical to dihydroorotase (Escherichia coli K-12 substr. MG1655)
Dihydroorotase. [EC: 3.5.2.3]

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.3

Use Curated BLAST to search for 3.5.2.3

Protein Sequence (344 amino acids)

>RALBFv3_RS11590 dihydroorotase (Ralstonia solanacearum IBSBF1503)
MIDTLTIVRPDDWHLHLRDGDALADVVGDTAHQFGRAIIMPNLKPPVTTTAQARTYRERI
LAALPAGTRFEPLMTLYLTDNTTPEEIRTARASGFVHGVKLYPAGATTNSDAGVTDLRRC
AKTLEAMQDVGMPLLVHGEVTDPTVDIFDREAVFIETVMQPLRRDFPALKVVFEHITTKH
AAEYVRDAQGPVGATITAHHLLYNRNALFVGGIRPHYYCLPVLKRETHRLALVAAATSGH
PRFFLGTDSAPHAKGLKEHACGCAGCYTALHAMELYAEAFEDANALDKLEGFASLHGPDF
YGLPRNAGTLTLTRSQWQLPAEVPFGEQTLVPLRGGEMLRWKTV