Protein Info for RALBFv3_RS10365 in Ralstonia solanacearum IBSBF1503

Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 24 to 137 (114 residues), 58.7 bits, E=6.1e-20
• PF02310: B12-binding
  • amino acids 26 to 129 (104 residues), 52.6 bits, E=8.5e-18
• PF03308: MeaB
  • amino acids 178 to 409 (232 residues), 141.2 bits, E=7e-45
• PF02492: cobW
  • amino acids 208 to 376 (169 residues), 31.1 bits, E=3.5e-11
• PF01642: MM_CoA_mutase
  • amino acids 570 to 1092 (523 residues), 419.5 bits, E=3.7e-129
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 730 to 1098 (369 residues), 306.4 bits, E=2.9e-95

Best Hits

Swiss-Prot: 82% identical to ICMF_CUPMC: Fused isobutyryl-CoA mutase (icmF) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K11942, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 75% identity to afw:Anae109_3169)

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (2/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• pyruvate fermentation to propanoate I (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (6/13 steps found)
• L-glutamate degradation VIII (to propanoate) (3/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (1099 amino acids)

>RALBFv3_RS10365 methylmalonyl-CoA mutase (Ralstonia solanacearum IBSBF1503)
MTDLSAARAAEPTSASAPGLVCNKVRFVTAASLFDGHDASINIMRRILQSMGCEVIHLGH
NRSVDEVVDAALQEDVQGIAVSSYQGGHVEYFKYMIDALRARGGERIQVFGGGGGVIVPA
EIRALQDYGVARIYSPEDGQRMGLAGMIADMVRRCDIDLSADAPATLEPVAAGDRRALAR
LITALESGRIDPALRQAVRERALTTPTPVLGVTGTGGAGKSSLTDELIRRFRLDQHDRLR
IAVISIDPSRRKSGGALLGDRIRMNAINHPHLFVRSMATREAASEIADALPDAIAACRAG
GFDLIIVETSGIGQGDAAIVPLVDLSLYVMTPEFGAASQLEKIDMLDFADLVAINKFDRK
GAQDAWRDVCKQVQRNREQWDAKPEAMPVFGTQASHFNDDGVTALYHALADRLAERGLAL
GERTLPRPPGPCSTSHDAIVPPARVRYLAEVADTVRGYHRRAEAQSRLARERQQLQASRR
MLEQAGAAGDALAALDTQAAERDARLGAAERKLLAMWPDLRRAYAGDEYVVKIRDREIRT
QLTHTTLSGTPLRKVVLPPYEDDGEILRWLLRENVPGSFPYTAGVFAFKRRGDVGGEDPT
RMFAGEGDAFRTNRRFKLVSEGMEAKRLSTAFDSVTLYGEDPDLRPDIYGKVGNAGVSIA
TLDDMRVLYDGFDLTSPNTSVSMTINGPAPTILAMFMNTAIDQNLDRFRADNSREPTEGE
EAKIRAWVLQNVRGTVQADILKEDQGQNTCIFSTAFSLKVMGDIQEYFVHHQVRNFYSVS
ISGYHIAEAGANPISQLAFTLANGFTYVEAYLARGMHIDDFAPNLSFFFSNGMDPEYCVL
GRVARRIWAVTMRDKYGANERSQKLKYHVQTSGRSLHAQEIAFNDIRTTLQALIAIYDNC
NSLHTNAYDEAITTPTTESVRRALAIQLIINREWGLAKCENPNQGSFIIDELTDLVEEAV
LQEFERLAERGGVLGAMETGYQRGKIQEESMLYEQRKHDGSLPIVGVNTFRNPEAGHAAP
AHIELARSSEEEKQRQLARLQDFHARHAAEAPAMLQRLQRAVIDDQNVFAVLMDAVRVCS
LGQITHALFEVGGQYRRNM