Protein Info for RALBFv3_RS09845 in Ralstonia solanacearum IBSBF1503

Annotation: multifunctional CCA addition/repair protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 PF01743: PolyA_pol" amino acids 22 to 145 (124 residues), 55 bits, see alignment E=1.6e-18 PF12627: PolyA_pol_RNAbd" amino acids 173 to 234 (62 residues), 40 bits, see alignment E=4.2e-14 PF01966: HD" amino acids 253 to 352 (100 residues), 34 bits, see alignment E=5e-12

Best Hits

Swiss-Prot: 94% identical to CCA_RALSO: Multifunctional CCA protein (cca) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 99% identity to rsc:RCFBP_21359)

MetaCyc: 55% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (429 amino acids)

>RALBFv3_RS09845 multifunctional CCA addition/repair protein (Ralstonia solanacearum IBSBF1503)
MTQRAASAGEAIVDPATHGLDVYAVGGAIRDTLLGLPVQDRDYVVVGATPAAMEARGFRT
VGKDFPVFLHPLTQAEYALARTERKTAAGYKGFSVYYAPDVTLEDDLVRRDLTINAMAQR
VAEDGALVGPVVDPYGGQADLAARAFRHVSEAFAEDPVRILRVARFAARFAEFHVAPETN
ALMQRMVEAGEVDALVPERVWQEFARGLMEARPSRMFAVLRDCGALARLLPELDRLWGVP
QRADYHPEIDTGVHTMMVVDTAAAMDTPLPVRFAALVHDLGKGTTPADILPRHVGHEARS
VPMIEDVCQRLRVPTDCRELAVVVAREHGNIHRSDGFDATALVRLLERCDALRKPERFRQ
ALLACEADARGRLGFEHRDYPQPARLLLALQAAVSIDAGAVAKRYADNPAHIKQAVHVAR
IEAVAQAGL