Protein Info for RALBFv3_RS08725 in Ralstonia solanacearum IBSBF1503

Annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 72 bits, see alignment (E = 5.4e-24)
• PF18327: PRODH
  • amino acids 101 to 148 (48 residues), 65.4 bits, see alignment (E = 1.1e-21)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 160 to 271 (112 residues), 152.3 bits, E=1.7e-48
• PF01619: Pro_dh
  • amino acids 280 to 580 (301 residues), 354 bits, E=1.7e-109
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 622 to 1125 (504 residues), 787.5 bits, E=2.3e-241
• PF00171: Aldedh
  • amino acids 673 to 1119 (447 residues), 361.8 bits, E=1.2e-111

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 68% identity to aav:Aave_3321)

MetaCyc: 72% identical to proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase (Pseudomonas putida KT2440)
RXN-14903 [EC: 1.5.5.2]; 1.5.5.2 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• ethene biosynthesis II (microbes) (4/4 steps found)
• L-N^δ-acetylornithine biosynthesis (6/7 steps found)
• L-arginine degradation I (arginase pathway) (3/3 steps found)
• L-proline degradation I (3/3 steps found)
• proline to cytochrome bo oxidase electron transfer (2/2 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Protein Sequence (1334 amino acids)

>RALBFv3_RS08725 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Ralstonia solanacearum IBSBF1503)
MATTTLGVKLDDASRERLKRAAQSIDRTPHWLIKQAIFTYLDQVERGQLPNDVKADANGP
AADGLPSAATAVAEMADADGTGSEAAVQPFLEFAQSVQPQSVLRAAITAAYRRPETEAIP
MLLEQARLPGALSSEARQLARDLAGKLRAQKVGAGREGLVQGLIQEFSLSSQEGVALMCL
AEALLRIPDKPTRDALIRDKISNGNWQSHLGQSPSLFVNAATWGLLLTGKLVATHNEAGL
SKALTRIIGKRGEPLIRKGVDMAMRLMGEQFVTGETISEALANARKYEAEGFRYSYDMLG
EAAMTEADAQRYLASYEQAIRAIGQASGGRGIYEGPGISIKLSALHPRYSRAQYDRTLNE
LYPRVKGLAMLAREYDIGINIDAEEADRLELSLDLLERLCFAPELAGWNGLGFVVQGYQK
RCPFVLDAIIDLARRSKHRLMIRLVKGAYWDSEVKRAQVDGLEGYPVYTRKVYTDVSYLA
CARKLLAAPDAVFPQFATHNAHTLAAIYHMAGQNYYPGQYEFQCLHGMGEPLYEQVVGGK
PGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSFVNRIADESIPLDALVADPVAVVE
AMHAEEGTLGLPHPKIPLPRQLYGDVRANSSGIDLANEQRLASLSSALLASTGMAWAAAP
TIGDAPYAGGSPQPVRNPADLRDVVGHVTEATPADVDAALTAAAAAAPIWQATPPEARAA
LLERAADRMEGQMQSLMGLIIREAGKTLPNAISEVREAVDFLRYYAAQVRGGFSNDTHRP
LGPVVCISPWNFPLAIFTGQVSAALAAGNPVLAKPAEQTPLIAAQAVRILREAGVPAGAV
QLLPGRGETVGAALVRDARTKGVMFTGSTEVARILQRTLAGRLDANGAPIPLIAETGGQN
AMIVDSSALAEQVVADVLSSAFDSAGQRCSALRVLCLQDDVADRVLAMLKGGMAELAMGN
PDRLATDVGPVIDAEARDNIAGHIDAMRAKGRRVHQAPLPAACAHGTFVPPTVIELDSLS
DLTREIFGPVLHVVRWKRTAKDTDGAGLTRLIEQINGTGYGLTLGIHTRIDETIAHIVER
AHVGNLYVNRNIVGAVVGVQPFGGEGLSGTGPKAGGPLYLLRLLSTCPQDAMRAALALTA
GAGTDIETDERRALLAPFDVLHDWARKQSPELAAQCDRLAAATATGAVLTLPGPTGERNT
YMLLPRDAVLCVAADPADWLRQLAAVLAVGSAAVVQENPAIAEVLRALPSAVQSRVRVVA
SLEDAAFDAVLHHGDSDHLRALCEGLARRAGPIVGVQGLPHGGQGLALERLLIERSLSVN
TAAAGGNASLMTIG