Protein Info for RALBFv3_RS06360 in Ralstonia solanacearum IBSBF1503

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 844 PF00359: PTS_EIIA_2" amino acids 8 to 143 (136 residues), 105 bits, see alignment E=8.7e-34 TIGR01003: phosphocarrier, HPr family" amino acids 162 to 238 (77 residues), 58.5 bits, see alignment E=5e-20 PF00381: PTS-HPr" amino acids 162 to 239 (78 residues), 80.5 bits, see alignment E=2e-26 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 274 to 828 (555 residues), 465.8 bits, see alignment E=1.8e-143 PF05524: PEP-utilisers_N" amino acids 276 to 394 (119 residues), 71.9 bits, see alignment E=1.3e-23 PF00391: PEP-utilizers" amino acids 424 to 494 (71 residues), 77.8 bits, see alignment E=1.1e-25 PF02896: PEP-utilizers_C" amino acids 521 to 805 (285 residues), 367 bits, see alignment E=1.7e-113

Best Hits

Swiss-Prot: 64% identical to PTFAX_XANCP: Multiphosphoryl transfer protein (fruB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 97% identity to rsc:RCFBP_10598)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (844 amino acids)

>RALBFv3_RS06360 phosphoenolpyruvate--protein phosphotransferase (Ralstonia solanacearum IBSBF1503)
MPSPLICAERIRLQQRATDKTGAIHAAGQLLADTGCIDPAYIDSLLRRETVANTFLGHGV
AIPHGMGEDRHLIRRTGIAVLQFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLN
DDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGCDLAEQLTLTLDYPSGLHARPAAQWV
ETARRFAARVQLRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALEALQHTIRN
LTAQERAQAARDTQAARTRQAARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGIPTVPD
HPVPLAAGGDLLDRALAGTRAELAALARDTAARLGDAEAGIFKAQAELLGDTDLMTLTCQ
TMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRDVGRRVLGHLDPALRGAAQTL
PDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLD
VASGTQTIVDGVGGQLYLDPDAASIAGANDWLREEAARAQREQAERGLPARTRDGHTVEI
AANVNQPAQAVEAVTLGAEGVGLMRTEFLFLERDHAPDEDEQYDVYAAMLDALGGRPLIV
RALDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLMEPQLRALYRAAGSGRPLSIMF
PMITSLDEVIALRAACERIRAELGAPAVPLGIMVEVPSAALLADRLAEHVDFFSIGTNDL
TQYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLT
GLGVHELSMSPRDIPAVKARLRDAHVRQLTELAGQALACASAEDVRALEATLPALRTGKE
MAAA