Protein Info for RALBFv3_RS06225 in Ralstonia solanacearum IBSBF1503

Annotation: preprotein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 PF07517: SecA_DEAD" amino acids 6 to 405 (400 residues), 440.2 bits, see alignment E=1e-135 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 825 (798 residues), 1122.2 bits, see alignment E=0 PF00270: DEAD" amino acids 99 to 216 (118 residues), 24.1 bits, see alignment E=8.2e-09 PF01043: SecA_PP_bind" amino acids 232 to 361 (130 residues), 132.7 bits, see alignment E=2.5e-42 PF21090: P-loop_SecA" amino acids 420 to 625 (206 residues), 316.9 bits, see alignment E=1.7e-98 PF07516: SecA_SW" amino acids 627 to 839 (213 residues), 224.2 bits, see alignment E=5.1e-70 PF02810: SEC-C" amino acids 914 to 932 (19 residues), 40 bits, see alignment (E = 8.6e-14)

Best Hits

Swiss-Prot: 75% identical to SECA_BURA4: Protein translocase subunit SecA (secA) from Burkholderia ambifaria (strain MC40-6)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 75% identity to bac:BamMC406_0497)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>RALBFv3_RS06225 preprotein translocase subunit SecA (Ralstonia solanacearum IBSBF1503)
MITGLLKKIFGSRNERLIKQYRRKVAQINALEPTFEALSDTELQAKTQEFRERFAKGETL
DALLPEAFAVCREAGKRVMKMRHFDMQLIGGMVLHDGKIAEMRTGEGKTLTATLPVYLNA
IAGQGVHVVTVNDYLAQRDAEWMGKLYNWLGLSVGVNLTTMAHDEKQAAYAADITYGTNN
EFGFDYLRDNMVYDGSQRVQRPLNYAIVDEVDSILIDEARTPLIISGQAEDHTDLYRRMN
SIPPLLTRQIGEEKADGTGVEKPGDYYVDEKSHQVYLTEAGHEKAEQILLQAGLLAEGES
LYAPQNITLMHHLYASLRAHSLFHRDQHYVVQSDEVIIVDEFTGRLMQGRRWSDGLHQAV
EAKEGVQIQQENQTLATITFQNYFRMYAKLAGMTGTADTEAYEFQEIYGLETVVIPTNRP
AQRKDLQDQIYKTSKERYDAVIRDIRDCYERGQPVLVGTTSIENSELLSHLLNQAQLPHQ
VLNAKQHAREAEIVAQAGRPKMITIATNMAGRGTDIVLGGNVEKQAGFVMVDESLSDDEK
AKRVKQLQDEWQSLHEQVKAAGGLHIVGTERHESRRIDNQLRGRAGRQGDPGSSRFYLSL
DDQLLRIFAGDRVRAIMDRLKMPEGEPIEAGIVTRSIESAQRKVEGRNFDIRKQLLQYDD
VANDQRREIYKLRNEILEAVDAGDLVKNLRESVFVELFRAYVPAESMEEQWDLAGLEKTL
RDDWGVEVPLAKTVEQAQSIEDEDLLTQVQEAVEAVYAGKVAQVGREAFAGFERSVMLQS
LDTHWREHLAALDHLRQGIHLRGYAQKDPKQEYKRESFELFGRLLDIVRNEVTRIIFTVR
IQSQEQLEEASEQIEEDLSHLTNVQYKHDEFSELAEVAAGDAEIHGETPALPAHRSAAAS
AAAALAGEVPKVGRNDPCPCGSGKKYKQCHGKLV