Protein Info for RALBFv3_RS05840 in Ralstonia solanacearum IBSBF1503

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 877 TIGR00396: leucine--tRNA ligase" amino acids 6 to 876 (871 residues), 1073.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 217 (204 residues), 87.5 bits, see alignment E=2.2e-28 amino acids 433 to 588 (156 residues), 32.7 bits, see alignment E=9.1e-12 amino acids 635 to 675 (41 residues), 26.2 bits, see alignment 8.8e-10 PF09334: tRNA-synt_1g" amino acids 44 to 177 (134 residues), 65.6 bits, see alignment E=9.2e-22 PF13603: tRNA-synt_1_2" amino acids 226 to 419 (194 residues), 223.4 bits, see alignment E=4.4e-70 PF08264: Anticodon_1" amino acids 717 to 839 (123 residues), 63.6 bits, see alignment E=4.9e-21

Best Hits

Swiss-Prot: 74% identical to SYL_BURCM: Leucine--tRNA ligase (leuS) from Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 74% identity to bam:Bamb_0549)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (877 amino acids)

>RALBFv3_RS05840 leucine--tRNA ligase (Ralstonia solanacearum IBSBF1503)
MQDKYSPSEVEQQAQQHWQAQDAYRVSEHARAADGSDKPKFYACSMLPYPSGKLHMGHVR
NYTINDVMTRHLRMKGYNVLMPMGWDAFGMPAENAALNNGVAPAAWTYDNIAYMKKQMQS
MGLAIDWSREVATCDPDYYRWNQWLFLKMLEKGIAYRKTGTVNWDPVDQTVLANEQVIDG
RGWRSGAVVEKREIPMYYLRITDYAQELLSDLDPLGWPERVKLMQQNWIGKSEGVRFAFP
HSIPGDDGKVIGDGKLYVFTTRADTIMGVTFCAVAAEHPIAAHAAQGNPALAAFIDECKH
GSVMEADMATMEKKGMPTGLTVTHPLTGEAVPVWVGNYVLMSYGDGAVMGVPAHDERDFA
FANKYGLPIKQVIDVKGQPYDTTTWAEWYGDKEHGVLFHSGKYDGLNYKQAVDAVAADLA
AQGLGEKKTTWRLRDWGISRQRYWGTPIPLIHCESCGVVPVPEQDLPVRLPEDLVPDGTG
NPLNKDSRFLSCTCPSCGKPARRETDTMDTFIDSCWYYMRYTCPDAGTMVDGRNDYWMPM
DQYIGGIEHAILHLLYARFWTKVMRDLGLVKFDEPFTNLLTQGMVLNETYYREDATGKKH
WINPADVDVQTDERGRPIGATLKADGQPVVIGGVEKMSKSKNNGIDPQALIDQYGADTAR
LFTMFAAPPEQQLEWNDAGVEGASRFLRRMWNFGVAHGDAIRAGHGNGVVDGATDADRAL
RRELHTVLKQANYDYERLQYNTVVSATMKMLNALEGAKDAGVDARREGFGILLRVLYPVV
PHITHVLWQELGYAGAYGSLLDAPWPQVDDGALVQSEIELVLQVNGKVRGSIVVPADADR
AAIEAIAAKDEAVHRFAEGKPPKKIIVVPGRLVNVVA