Protein Info for RALBFv3_RS05700 in Ralstonia solanacearum IBSBF1503

Annotation: protein translocase subunit SecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 141 to 159 (19 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 193 to 215 (23 residues), see Phobius details amino acids 250 to 297 (48 residues), see Phobius details PF07549: Sec_GG" amino acids 32 to 59 (28 residues), 31.7 bits, see alignment (E = 8.1e-12) TIGR00966: protein-export membrane protein SecF" amino acids 38 to 288 (251 residues), 244.8 bits, see alignment E=1.2e-76 PF02355: SecD_SecF" amino acids 114 to 296 (183 residues), 214.7 bits, see alignment E=8e-68 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 120 to 285 (166 residues), 160.9 bits, see alignment E=3.8e-51

Best Hits

Swiss-Prot: 40% identical to SECF_VIBAL: Protein translocase subunit SecF (secF) from Vibrio alginolyticus

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 99% identity to rsc:RCFBP_10734)

MetaCyc: 39% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (322 amino acids)

>RALBFv3_RS05700 protein translocase subunit SecF (Ralstonia solanacearum IBSBF1503)
MEFFRIRRDIPFMKHALAFNAVSFLTFIAAVFFLFHNGLHLSVEFTGGTVMEVAYTQAAD
LPKVRADVEKLGYADAQVQNFGTSRDVMIRLPLKNGPDGKPIASAVQSQQVMSALTADTP
DVKLQRVEFVGPQVGKELATDGLLALLFVVIGIVIYLSIRFEWKFAVAGIIANLHDIVII
LGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVIFDRIREAFRKYRKMTTHEVIDHAITST
ISRTIITHGSTEMMVLSMLVFGGPTLHYFALALTIGILFGIYSSVFVAAALCMWLGVKRE
DLVKGEKKAAGQKDDPNFGAQV