Protein Info for RALBFv3_RS05395 in Ralstonia solanacearum IBSBF1503

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 PF17391: Urocanase_N" amino acids 22 to 148 (127 residues), 203.4 bits, see alignment E=1.4e-64 PF01175: Urocanase" amino acids 151 to 174 (24 residues), 23.3 bits, see alignment (E = 6.7e-09) amino acids 198 to 394 (197 residues), 233.9 bits, see alignment E=2.5e-73 TIGR01228: urocanate hydratase" amino acids 201 to 599 (399 residues), 642 bits, see alignment E=4.2e-197 PF17392: Urocanase_C" amino acids 397 to 591 (195 residues), 310.3 bits, see alignment E=6.8e-97

Best Hits

Swiss-Prot: 94% identical to HUTU_RALSO: Urocanate hydratase (hutU) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 97% identity to rsc:RCFBP_10800)

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (601 amino acids)

>RALBFv3_RS05395 urocanate hydratase (Ralstonia solanacearum IBSBF1503)
MNTMTDAAHLAADPRYDASREIRAPRGTELHCKSWLTEAAYRMLQNNLDPDVAENPKHLV
VYGGIGRAARDWACFDKILETLRTLNDDESLLVQSGKPVGVFRTHPDAPRVLIANSNLVP
KWADWAHFNELDRKGLFMYGQMTAGSWIYIGSQGIVQGTYETFAEAGRQHYSDRPSALLK
PGRPPEGTAPGSERPGTQVPGGNLAGRWILTAGLGGMGGAQPLAATLAGAVSLTIECQQS
SIDFRLRTRYLDKQAKDIDDALNLIRHHCERGEAVSIGLLGNAAEILPELVKRARAGGLK
PDLVTDQTSAHDLVNGYLPVGWTVEQWRAAQRDPSQHARLTEAAAGSCAVHVQAMLDFQA
MGIPTVDYGNNIRQVAFDRGVKNAFDFPGFVPAYIRPLFCEGRGPFRWVALSGDPEDIYK
TDAKIKALFPHNTHVHRWLDMARERIAFQGLPARICWLGLGERHLAGLAFNEMVRTGELK
APVVIGRDHLDTGSVASPNRETEAMRDGTDAVSDWPLLNALLNTAGGATWVSLHHGGGVG
MGYSQHAGVVIVADGTEAAARRLARVLVNDAGSGVMRHADAGYETAIACAKRNGLKLPMI
G