Protein Info for RALBFv3_RS05200 in Ralstonia solanacearum IBSBF1503

Annotation: murein biosynthesis integral membrane protein MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 transmembrane" amino acids 15 to 33 (19 residues), see Phobius details amino acids 97 to 127 (31 residues), see Phobius details amino acids 143 to 165 (23 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 252 to 272 (21 residues), see Phobius details amino acids 292 to 310 (19 residues), see Phobius details amino acids 331 to 352 (22 residues), see Phobius details amino acids 372 to 392 (21 residues), see Phobius details amino acids 402 to 422 (21 residues), see Phobius details amino acids 428 to 447 (20 residues), see Phobius details amino acids 459 to 481 (23 residues), see Phobius details amino acids 495 to 518 (24 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 16 to 522 (507 residues), 466 bits, see alignment E=8.1e-144 PF03023: MurJ" amino acids 41 to 487 (447 residues), 451 bits, see alignment E=2.1e-139

Best Hits

Swiss-Prot: 64% identical to MURJ_BURCJ: Lipid II flippase MurJ (murJ) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K03980, virulence factor (inferred from 98% identity to rsc:RCFBP_10841)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (530 amino acids)

>RALBFv3_RS05200 murein biosynthesis integral membrane protein MurJ (Ralstonia solanacearum IBSBF1503)
MPARSGAPTTHSTLNLLRTLATISGLTMLSRITGLIRETLIARAFGASVYTDAFNVAFRI
PNLLRRLSAEGAFSQAFVPILGEFKNRHGEAQTRALVDAVATVMTWFLVVISALGVIGAP
LIVAAVATGFKTHESQAYISAVFMTRVMFPYIGLVSLVALASGILNTWRQFGIPAFTPVL
LNLSFIVAAVFVAPMLQTPIYAQAYAVMVGGILQLAIQIPSLRRMGMLPRVSLDMRAAWH
HPGVRRVLKQMLPATLSVSVAQVSLIINTNIASRLPAGSVSWLNYADRLMEFPTALLGVA
LGTILLPSLSKASAEEHREEYSSLLDWGLRLTVLLAVPSAVGLFVFGAPLTATLFHYGRF
NGLDVEMTRQALVSYGVGLIGLIVIKILAPGFYARQDIRTPVKIALVVLAVTQLSNIVFV
PMFGHAGLALSISFGATINATLLFLGLRRRGYYHPLPGWGLFLAQVTAAVLLLSGVLLWF
AQTFDWVGMGARPLMRVTLLGACLVLCAVVYFGTLWLTGLKFSTFRKRAI