Protein Info for RALBFv3_RS05060 in Ralstonia solanacearum IBSBF1503

Annotation: exodeoxyribonuclease VII large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 PF13742: tRNA_anti_2" amino acids 30 to 123 (94 residues), 105.5 bits, see alignment E=2.2e-34 TIGR00237: exodeoxyribonuclease VII, large subunit" amino acids 31 to 376 (346 residues), 425 bits, see alignment E=1.6e-131 PF01336: tRNA_anti-codon" amino acids 51 to 123 (73 residues), 47.5 bits, see alignment E=2.1e-16 PF02601: Exonuc_VII_L" amino acids 146 to 456 (311 residues), 341.8 bits, see alignment E=7.2e-106

Best Hits

Swiss-Prot: 96% identical to EX7L_RALSO: Exodeoxyribonuclease 7 large subunit (xseA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K03601, exodeoxyribonuclease VII large subunit [EC: 3.1.11.6] (inferred from 97% identity to rsc:RCFBP_10868)

MetaCyc: 43% identical to exodeoxyribonuclease VII subunit XseA (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)" in subsystem DNA repair, bacterial (EC 3.1.11.6)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.6

Use Curated BLAST to search for 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (468 amino acids)

>RALBFv3_RS05060 exodeoxyribonuclease VII large subunit (Ralstonia solanacearum IBSBF1503)
MDTPAAASATPSAVPAPPPAAPVRGGQEVLSVSEVNRRIAGLLERHFALGWVRGEISNFT
RAGSGHWYFSLKDAGAQIRCVMFKGRNQYADFTPREGEAVEVRAVVTLYEARGELQLSVE
AIRRAGLGNLYEAFLRLKAALEAQGLFDAARKRPLPRHPHAIGVVTSLQAAALRDVLTTL
RRRAPHVPVAVYPVPVQGAGAAERIAAMLDIVNARAEVDVIILCRGGGSIEDLWAFNEES
VARAVAASDIPIVAGVGHETDVTIVDFVADVRAPTPTGAAELVSPDRARMLRDVALCWDA
LSATLRRQLDRRAQTVDWLARRLRSPQAQMRERRTWLAHLEGRLRFALHGRVQRAEHVQR
LLAMRLAAARPDTARERTALQGIEAALARAMRATLDRAQTRLSHRQAGLELLAPQRTLER
GYAVLLDADGRAIRDPNALHARARIEARLALGAVDIEIAQAQPKLPDM