Protein Info for RALBFv3_RS04810 in Ralstonia solanacearum IBSBF1503

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 762 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 735 (733 residues), 1105.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 50.5 bits, see alignment 1.1e-16 PF00004: AAA" amino acids 212 to 344 (133 residues), 45 bits, see alignment E=9e-15 amino acids 493 to 616 (124 residues), 48.4 bits, see alignment E=8.2e-16 PF17871: AAA_lid_9" amino acids 351 to 453 (103 residues), 93.6 bits, see alignment E=3.9e-30 PF07724: AAA_2" amino acids 487 to 647 (161 residues), 202.9 bits, see alignment E=2.1e-63 PF00158: Sigma54_activat" amino acids 491 to 613 (123 residues), 22.5 bits, see alignment E=4.8e-08 PF07728: AAA_5" amino acids 492 to 610 (119 residues), 51.6 bits, see alignment E=6.2e-17 PF10431: ClpB_D2-small" amino acids 654 to 734 (81 residues), 81.3 bits, see alignment E=2.5e-26

Best Hits

Swiss-Prot: 64% identical to CLPA_ECOL6: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 88% identity to bml:BMA10229_A1053)

MetaCyc: 64% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (762 amino acids)

>RALBFv3_RS04810 ATP-dependent Clp protease ATP-binding subunit ClpA (Ralstonia solanacearum IBSBF1503)
MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPTAAEVLRACAANIEDLRTHLKN
FIADNTPVVPGTDEVDTQPTLGFQRVIQRAIMHVQSTSNGKKEVTGANVLVAIFGEKDSH
AVYYLQQQGVTRLDVVNFISHGIRKDQAEPAKHGDSNPEGEAGDGKESPLEQFTQNLNAL
AKAGKIDPLIGREQEVERVVQVLCRRRKNNPLLVGEAGVGKTAIAEGLAWRITKGEVPDI
LARSVVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDNPNAILFIDEIHTLIGAGAASGG
TLDASNLLKPALSSGALKCIGATTFTEYRGIFEKDAALSRRFQKIDVVEPSVDQTVQILR
GLKSRFEEHHGVKYAAAALTAAAELSARFITDRHLPDKAIDVIDEAGAAQRILPKSKQKK
TIGKGEIEDIVSRIARVPPQSVSQDDRSKLQTLERDLKSVVFGQDPAIDALASAIKMSRA
GLGKTDKPIGSFLFSGPTGVGKTEVAKQLAFILGIELIRFDMSEYMERHAVSRLIGAPPG
YVGFDQGGLLTEAITKKPHCVLLLDEIEKAHPDIFNILLQVMDHGALTDNNGRKADFRNV
IIIMTTNAGAETMNKATIGFTTAREQGDEMADIKRMFTPEFRNRLDATISFRSLDEEIIL
RVVDKFLMQLEEQLHEKKVDAIFTEKLRHFLAKKGFDPLMGARPMQRLIQDMIRKALADE
LLFGKLVNGGKVVVDLDDADTVKLEFSENEAPPAPAQEEAEA