Protein Info for RALBFv3_RS04525 in Ralstonia solanacearum IBSBF1503
Annotation: prolipoprotein diacylglyceryl transferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to LGT_RALSO: Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase (lgt) from Ralstonia solanacearum (strain GMI1000)
KEGG orthology group: K13292, phosphatidylglycerol:prolipoprotein diacylglycerol transferase [EC: 2.-.-.-] (inferred from 98% identity to rsc:RCFBP_11016)MetaCyc: 54% identical to phosphatidylglycerol--prolipoprotein diacylglyceryl transferase (Escherichia coli K-12 substr. MG1655)
RXN0-20 [EC: 2.5.1.145]
Predicted SEED Role
"Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-)" (EC 2.4.99.-)
MetaCyc Pathways
- lipoprotein posttranslational modification (Gram-negative bacteria) (3/7 steps found)
KEGG Metabolic Maps
- Glycosphingolipid biosynthesis - ganglio series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Lipopolysaccharide biosynthesis
- Puromycin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.-.-.-
Use Curated BLAST to search for 2.-.-.- or 2.4.99.- or 2.5.1.145
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (303 amino acids)
>RALBFv3_RS04525 prolipoprotein diacylglyceryl transferase (Ralstonia solanacearum IBSBF1503) MLIHPQFDPVALHLGPLAIRWYGLMYLAAFIMFLWFGRLRTRQSHIAAQGWRGSDLDDML FYGVLGVILGGRLGYVLFYKPDWYLAHPLDIFKVWEGGMAFHGGFLGVVLAMVLYARMRR RPWTQVTDFIAPMVPCGLAAGRLGNFINGELWGRVASPDLSWAMLFPQAQGEDRAWLASH AQQAVASGVQAVFDQYHMLPRHPSQIYQFLGEGVLFFILLWLYARKPRPMGAVSGMFLIG YGVFRFAAEFAREPDNFLGLLALNLSMGQWLSLPMILAGAAMLAWAYRRAGRNGNDAQAS AHA