Protein Info for RALBFv3_RS03315 in Ralstonia solanacearum IBSBF1503

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 TIGR00691: RelA/SpoT family protein" amino acids 113 to 802 (690 residues), 738.9 bits, see alignment E=2.9e-226 PF13328: HD_4" amino acids 113 to 262 (150 residues), 176.4 bits, see alignment E=1.1e-55 PF01966: HD" amino acids 132 to 231 (100 residues), 41.8 bits, see alignment E=3.7e-14 PF04607: RelA_SpoT" amino acids 322 to 432 (111 residues), 133.6 bits, see alignment E=1.1e-42 PF02824: TGS" amino acids 475 to 534 (60 residues), 71.6 bits, see alignment 1.3e-23 PF19296: RelA_AH_RIS" amino acids 547 to 696 (150 residues), 34.2 bits, see alignment E=8.2e-12 PF13291: ACT_4" amino acids 724 to 803 (80 residues), 57 bits, see alignment E=7.8e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 96% identity to rsl:RPSI07_1272)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (808 amino acids)

>RALBFv3_RS03315 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Ralstonia solanacearum IBSBF1503)
MPTSASGSASPRTGAPNLPDPHGADLVGERAALPPRSAKAAPSGADVSVPAATEPHASET
LFIDAVLEQTYRHLFGPTSQPAAPPRQQVVSIAGLTEKLSYLKPADMKLVKEAFHFSDEA
HLGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGAKV
AELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPE
KRRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVV
SRILEAAQRALADAGIPAELTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVETQMQC
YMAVGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTREMNQIAEA
GVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQSGDPQEFLEHVKIDLFPDAVYVFTP
KGEIRALPRGATALDFAYAVHSDLGNQCVAVKINNALLPLRTELKNGDIVEVVTAPYSKP
NPAWLTFVRTGKARAAIRHFLKTAKLDEAIQLGERLLEQALRQLGIDMKAVPAPVWERIV
QWTGNKAREDIFADLALGRRVAAVVARRTEIVLQEGAYEGDEALIAAVHTFSGEEAPAVT
VSGDEGMAMVFSPCCRPIPGDPIVGYIGKGEGLQIHVEECRVAKRLHGKDPEHWIDVMWA
DHTTRAFDVSIKVLVHNTKGILARVAADLTSADANVAHVSMEQEGGNQEATYMTFLIQVH
DRVHLADVMRVLRRNPDVIRIARDRGGE