Protein Info for RALBFv3_RS03130 in Ralstonia solanacearum IBSBF1503

Annotation: tungsten ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF12849: PBP_like_2" amino acids 23 to 249 (227 residues), 143.1 bits, see alignment E=1.5e-45 PF13531: SBP_bac_11" amino acids 40 to 257 (218 residues), 47.7 bits, see alignment E=1.8e-16

Best Hits

Swiss-Prot: 46% identical to TUPA_PEPAC: Tungstate-binding protein TupA (tupA) from Peptoclostridium acidaminophilum

KEGG orthology group: K05772, putative tungstate transport system substrate-binding protein (inferred from 97% identity to rsc:RCFBP_11296)

Predicted SEED Role

"ABC-type tungstate transport system, periplasmic binding protein" in subsystem ABC transporter tungstate (TC 3.A.1.6.2)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (278 amino acids)

>RALBFv3_RS03130 tungsten ABC transporter substrate-binding protein (Ralstonia solanacearum IBSBF1503)
MSKVSAILRFYTALCMAFAVAGTAHAGDLRMATTTSTENSGLLKVLLPKFEAATGVHVQV
IAVGTGKAVKLGEAGDVDVVLVHARPLEDAFVASGGGIDRRDVMYNDFILVGPTADPAHV
RAQKNVIQGMEAIAAAGAKFVSRGDNSGTDVMEQRYWKTAGIDPKGKPWYVSTGLGMGEV
LNMAAQMDAYTLSDRATYGAYRARTGLDIVLAGDPRMFNPYGIIAVNPARHPGINYADAT
RLIEWITSSAGQQAIAEFKVNGEQVFFPNYGKALAGGK