Protein Info for RALBFv3_RS03050 in Ralstonia solanacearum IBSBF1503

Annotation: acetolactate synthase 3 large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 19 to 579 (561 residues), 843.8 bits, E=2.9e-258
• PF02776: TPP_enzyme_N
  • amino acids 19 to 135 (117 residues), 147.3 bits, E=2.6e-47
• PF00205: TPP_enzyme_M
  • amino acids 209 to 344 (136 residues), 168.8 bits, E=8.2e-54
• PF02775: TPP_enzyme_C
  • amino acids 408 to 556 (149 residues), 182.4 bits, E=7.1e-58

Best Hits

Swiss-Prot: 48% identical to ILVB_MYCLE: Acetolactate synthase (ilvB) from Mycobacterium leprae (strain TN)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 100% identity to rsl:RPSI07_1351)

MetaCyc: 90% identical to acetohydroxyacid synthase subunit B (Cupriavidus necator H16)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to (S)-acetoin (3/3 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• L-isoleucine biosynthesis II (6/8 steps found)
• pyruvate fermentation to (R)-acetoin I (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• superpathway of 2,3-butanediol biosynthesis (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of (R,R)-butanediol biosynthesis (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Protein Sequence (585 amino acids)

>RALBFv3_RS03050 acetolactate synthase 3 large subunit (Ralstonia solanacearum IBSBF1503)
MNMPSAEFSHANSGSSADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEH
ILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPT
HAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKAFYIASTGRPGPVVVDIPKDV
SRDMCKYEYPKTIEMRSYNPVNKGHSGQIRKAVSLLLQAERPYIYTGGGVVLANASEELR
QLAALTGYPVTNTLMGLGSFPGTSKQFVGMLGMHGTYEANMAMQHCDVLIAVGARFDDRV
IGSPAHFASQPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQEMIAQIQAGEAKPNKAA
LAKWWEQIEQWRSIDCLKYDRTSEIIKPQYVVEKIWELTAGDAFICSDVGQHQMWAAQFY
KFNEPRRWINSGGLGTMGVGLPYAMGVKKAFPDKEVVTITGEGSIQMCIQELSTCLQYNT
PVKIAALNNRYLGMVRQWQEIEYDNRYSHSYMEALPDFVKLAEAYGHVGMRIEKTADVEP
ALREAFKLKDRTVFMDFQTDPTENVWPMVQAGKGISEMLLGAEDL