Protein Info for RALBFv3_RS02940 in Ralstonia solanacearum IBSBF1503

Annotation: NADH-quinone oxidoreductase subunit H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00146: NADHdh
  • amino acids 26 to 343 (318 residues), 412.2 bits, E=6.7e-128

Best Hits

Swiss-Prot: 99% identical to NUOH_RALSO: NADH-quinone oxidoreductase subunit H (nuoH) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00337, NADH dehydrogenase I subunit H [EC: 1.6.5.3] (inferred from 99% identity to rso:RSc2055)

MetaCyc: 37% identical to ferredoxin-quinone oxidoreductase subunit A (Parasynechococcus marenigrum WH 8102)
RXN-22408 [EC: 7.1.1.10]; Carbonate dehydratase. [EC: 7.1.1.10, 4.2.1.1]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• cyanate degradation (2/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) (2/4 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• cyclic electron flow (1/3 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
• Fe(II) oxidation (2/6 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism
• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3, 4.2.1.1

Use Curated BLAST to search for 1.6.5.3 or 4.2.1.1 or 7.1.1.10

Protein Sequence (354 amino acids)

>RALBFv3_RS02940 NADH-quinone oxidoreductase subunit H (Ralstonia solanacearum IBSBF1503)
MIESITSFGAATFGGWWPLIWTLVRAVCIILPLLLCVAYLILWERKLIGWMHVRVGPNRV
GPMGLLQPIADVLKLLLKEVMVPSAVSRGMYIIAPLMVLMPAVAIWAVVPFQAEAVVSNI
NAGLLYVMAISSVGVYGVILAGWASNSKYAFLGAMRASAQMISYEIAMGFALVTVLMVTG
SLNLSDIVNSQNRGFFANHGINILSWNWLSLLPMFGVYFISGVAETNRHPFDVVEGESEI
VAGHMIEYSGMAFALFFLAEYINMIVISAMTATMFLGGWASPIDAPVFNWIPGFFWLLIK
VFLLLSVFIWLRASFPRYRYDQIMRLGWKIFIPLTVGWLMVVAIWLVSPWNIWK