Protein Info for RALBFv3_RS02190 in Ralstonia solanacearum IBSBF1503

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1081 TIGR01369: carbamoyl-phosphate synthase, large subunit" amino acids 2 to 1061 (1060 residues), 1542.7 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 128 to 341 (214 residues), 238.6 bits, see alignment E=1.8e-74 amino acids 678 to 884 (207 residues), 124.5 bits, see alignment E=1.6e-39 PF02787: CPSase_L_D3" amino acids 429 to 507 (79 residues), 82.8 bits, see alignment 4.9e-27 PF02222: ATP-grasp" amino acids 687 to 854 (168 residues), 32.7 bits, see alignment E=1.9e-11 PF02142: MGS" amino acids 965 to 1051 (87 residues), 79.3 bits, see alignment E=6.9e-26

Best Hits

Swiss-Prot: 60% identical to CARB_ARCFU: Carbamoyl-phosphate synthase large chain (carB) from Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 68% identity to abn:AB57_3102)

MetaCyc: 69% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1081 amino acids)

>RALBFv3_RS02190 carbamoyl-phosphate synthase large subunit (Ralstonia solanacearum IBSBF1503)
MPKRTDIKTILIIGAGPIIIGQACEFDYSGAQACKALREEGFKVVLVNSNPATIMTDPST
ADVTYIEPITWEVVERIIAKERPDAILPTMGGQTALNCALDLHRHGVLQKYNVELIGASP
EAIDKAEDRQKFKDAMTKIGLGSAKSGIAHSLEEALAVQAQIARETGTSGYPIVIRPSFT
LGGTGGGIAYNREEFEDICKRGLDLSPTNELLIEESLLGWKEYEMEVVRDKKDNCIIVCS
IENLDPMGIHTGDSITVAPAQTLTDKEYQILRNASLAVLREIGVDTGGSNVQFSINPQDG
RMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELKNEITGGATPASFEPSID
YVVTKVPRFAFEKFPQADSHLTTQMKSVGEVMAMGRTFQESFQKALRGLEVGVDGLDEKS
TDRDEIIEEIGEAGPDRIWYLGDAFRLGLSIDEVYAETAVDPWFLAQIEDIVKTEALVKA
RTLESLSAAELRLLKQKGFSDRRLAKLMKTEARAVREKRIAEKVRPVYKRVDTCAAEFAT
NTAYLYSTYEAEHGECEAEPTDRKKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYET
IMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVDKEKPVGVIVQYGGQTPLKLALDLEA
NGVPIIGTTPDMIDAAEDRERFQKLLHELGLRQPPNRTARAEDEALKLAEEIGYPLVVRP
SYVLGGRAMEIVHEPRDLERYMREAVKVSNDSPVLLDRFLNDAIECDVDCLSDGKRVFIG
GVMEHIEQAGVHSGDSACSLPPYSLSQATVDELKRQTAAMAKALNVVGLMNVQFAIQQKG
GEDIVYVLEVNPRASRTVPYVSKATGISLAKVAARCMAGQSLDEQGINHEVVPSYYSVKE
AVFPFNKFPGVDPVLGPEMRSTGEVMGVGRTFGEALFKSQLAAGSRLPEKGTVLMTVKDS
DKPRAIEVARTLHTLGYPIVATRGTASAIEAAGIPVRVVNKVKDGRPHIVDMIKNGELAL
VFTTVDETRAAIADSRSIRTAALANRVTYYTTIAGARAAVEGLKHLQNLDVYDLQGLHAS
L