Protein Info for RALBFv3_RS01505 in Ralstonia solanacearum IBSBF1503

Annotation: class I poly(R)-hydroxyalkanoic acid synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01838: poly(R)-hydroxyalkanoic acid synthase, class I
  • amino acids 73 to 596 (524 residues), 763.9 bits, E=3.8e-234
• PF07167: PhaC_N
  • amino acids 107 to 273 (167 residues), 230.4 bits, E=1.2e-72
• PF00561: Abhydrolase_1
  • amino acids 249 to 520 (272 residues), 113.3 bits, E=1.5e-36

Best Hits

Swiss-Prot: 73% identical to PHAC_CUPNH: Poly(3-hydroxyalkanoate) polymerase subunit PhaC (phaC) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K03821, polyhydroxyalkanoate synthase [EC: 2.3.1.-] (inferred from 99% identity to rsc:RCFBP_11639)

MetaCyc: 73% identical to poly-beta-hydroxybutyrate polymerase subunit (Cupriavidus necator)
RXN1-42 [EC: 2.3.1.304]

Predicted SEED Role

"Polyhydroxyalkanoic acid synthase" in subsystem Polyhydroxybutyrate metabolism

MetaCyc Pathways

• polyhydroxybutanoate biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.3.1.304

Protein Sequence (598 amino acids)

>RALBFv3_RS01505 class I poly(R)-hydroxyalkanoic acid synthase (Ralstonia solanacearum IBSBF1503)
MASRHKASSHASGSQAQPTWQGLADPAQWAEQFRQWQSMAGAFAQSGAPGSAGFGPVNGM
PAGEGAIPFALIPPERLAEIQQRYLEAWLQIWRHMSAGDSAETVAPSDRRFANDAWRKSP
LYGYAAAFYVLNARTLMEMAEAVQADAKTRERVRFAVSQWTAAMSPSNFLATNPEAQRQL
IESRGESLRTGILNMLQDMERGKISQTDETAFEIGRNVANSEGSVVFENDYFQLIQYKPL
TAKVHARPLLLVPPCINKYYILDLQPANSLVRYSVEQGHTVFLVSWRNPDASMAARNWDD
YIEGGAIEAIRVAREISAQDQINVLGFCVGGTILSTALAVLAARGQHPAASLTLLTTLLD
FSDTGILDVFVDQAQVEMREQTIGSAAPAGPRLLRGVELANTFSFLRPNDLVWNYVVENY
LKGKTPAPFDLLYWNGDSTNLPGPWYCWYLRHTYLQNDLMVPGKLTVCGEPIDLGRIDVP
VYLYGSREDHIVPWKSAYASTQLLKGKLRFVLGASGHIAGVINPPAANKRSHWINAKLAE
SADAWLEGAQEHPGSWWPDWSAWLATHAGVQKAAPKRYGNADHPAIEPAPGRYVKQKA