Protein Info for RALBFv3_RS00410 in Ralstonia solanacearum IBSBF1503

Annotation: RIP metalloprotease RseP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 102 to 126 (25 residues), see Phobius details amino acids 386 to 405 (20 residues), see Phobius details amino acids 432 to 451 (20 residues), see Phobius details TIGR00054: RIP metalloprotease RseP" amino acids 2 to 458 (457 residues), 309.2 bits, see alignment E=2.2e-96 PF02163: Peptidase_M50" amino acids 6 to 445 (440 residues), 229 bits, see alignment E=9.2e-72 PF00595: PDZ" amino acids 229 to 265 (37 residues), 25.1 bits, see alignment 3.7e-09 PF17820: PDZ_6" amino acids 233 to 285 (53 residues), 33.4 bits, see alignment 6.3e-12

Best Hits

Swiss-Prot: 96% identical to Y1411_RALSO: Putative zinc metalloprotease RSc1411 (RSc1411) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K11749, regulator of sigma E protease [EC: 3.4.24.-] (inferred from 98% identity to rsc:RCFBP_20022)

Predicted SEED Role

"Membrane-associated zinc metalloprotease" in subsystem Sex pheromones in Enterococcus faecalis and other Firmicutes

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>RALBFv3_RS00410 RIP metalloprotease RseP (Ralstonia solanacearum IBSBF1503)
MLTVLAFVFAIAVLIVVHELGHYSVARLCGVKVLRFSVGFGKVLFRRVGRGPDRTEWTIC
AIPLGGYVKMLGESARDPERDPPILPEDLPRTFDHQPVYKRFAIVAAGPVFNFLLAIVLY
ALLAWVGAQEPLPILGAPPPGSIAAQADLRAKDRVVAVGTDDEAPTPVRAWSDVRMRLYE
AGIGGRDAIVQVRGADGAERTARLRGLPSAARSPQADVIDQIGLRLLGGPVTIAEVLPGG
AGERAGLHRGDQIVRFAGQPADQASDLIRWIRAMPEQNASIDILRGGQPMTLPVRLGADA
DPANPSGAKIGKLGAQLSQHVETELIRDEPVQAWVHAMREVWRTSMLSLKVLGKMIVGQA
SLQNLSGPITVADFAGKAASLGWQSFVGFLALISVSLGVLNLLPVPVLDGGHLLYYCVEF
LTGKPVPESWQAVLQKIGIACILLLTSLALYNDLSRLFLAHG