Protein Info for Psyr_5019 in Pseudomonas syringae pv. syringae B728a
Annotation: Acetyl-CoA hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 51% identical to CAT1_CLOK5: Succinyl-CoA:coenzyme A transferase (cat1) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
KEGG orthology group: None (inferred from 100% identity to psb:Psyr_5019)MetaCyc: 51% identical to succinyl-CoA:CoA transferase (Clostridium kluyveri)
RXN-8807 [EC: 2.8.3.18]
Predicted SEED Role
"Propionyl-CoA:succinyl-CoA transferase"
MetaCyc Pathways
- TCA cycle VII (acetate-producers) (8/9 steps found)
- pyruvate fermentation to acetate V (3/3 steps found)
- acetate formation from acetyl-CoA (succinate) (1/1 steps found)
- pyruvate fermentation to acetate VI (2/3 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (11/17 steps found)
- succinate fermentation to butanoate (1/7 steps found)
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.8.3.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZLC6 at UniProt or InterPro
Protein Sequence (497 amino acids)
>Psyr_5019 Acetyl-CoA hydrolase (Pseudomonas syringae pv. syringae B728a) MYRDRIRRPSLMSKVMSAADAAALIEDGMTVGMSGFTRAGEAKAVPHALAERAKVTPLKI SLMTGASLGNDLDKQLTESGVLSRRMPFQVDSTLRKAINNGTVMFIDQHLSDTVELLRNR QIKAVDLAVIECVAITEEGHLVLSTSVGNSASFAILAKQVIVEINLSQPLELEGLHDIYI PSYRPTRLPIPVLEADSRIGSHAVKIDPAKIVGIVISNQPDSPSTVTPPDADTQAIANHL VEFFKKEVREDRLTDKLMPLQAGIGNIANAVMHGLLASPFTDLTMYSEVLQDSTFDLFDA GKLTFASGSSMTLSAAKHAEVFADFNRYKSRLVLRPQEISNHPEVIRRLGIIGINTALEF DLYGNVNSTHICGTRMMNGIGGSGDFSRNAHLAIFVTKSIAKGGAISSVVPMVSHVDHTE HDVDILVTEQGLADLRGLAPRERARVIIDNCVHPDYRDALNAYLSAACAVGGHTPHILRE ALSWHINLEETGRMLPA