Protein Info for Psyr_5018 in Pseudomonas syringae pv. syringae B728a

Annotation: NAD(P) transhydrogenase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details amino acids 24 to 24 (1 residues), see Phobius details transmembrane" amino acids 22 to 23 (2 residues), see Phobius details amino acids 35 to 56 (22 residues), see Phobius details amino acids 66 to 86 (21 residues), see Phobius details amino acids 97 to 118 (22 residues), see Phobius details amino acids 139 to 162 (24 residues), see Phobius details amino acids 182 to 201 (20 residues), see Phobius details amino acids 207 to 227 (21 residues), see Phobius details amino acids 233 to 251 (19 residues), see Phobius details amino acids 257 to 276 (20 residues), see Phobius details PF02233: PNTB" amino acids 7 to 479 (473 residues), 626.7 bits, see alignment E=1.4e-192

Best Hits

Swiss-Prot: 62% identical to PNTB_RHORT: NAD(P) transhydrogenase subunit beta (pntB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 100% identity to psb:Psyr_5018)

Predicted SEED Role

"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLC7 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Psyr_5018 NAD(P) transhydrogenase, beta subunit (Pseudomonas syringae pv. syringae B728a)
MSMNLVTLLYLIASICFIQALKGLSHPTTSRRGNLFGMLGMGLAVITTIGLVFKLAALSS
AEGTSAGIGYIVVGLLVGGTAGSIMAKRVEMTKMPELVAFMHSMIGLAAVFIAIAAVVEP
QSLGIVRNLGDAIPAGNRLELFLGAAIGAITFSGSVIAFGKLSGKYKFRLFQGAPVQFAG
QHTLNLVLGLATLFFGLTFMFTGSLTAFAIMLALAFVIGVLLIIPIGGADMPVVVSMLNS
YSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSLFNVIGGGFGGATDSAG
PAGAKEARPVKSGSADDATFLLTNADTVIIVPGYGLAVARAQHALKELTEKLVHRGVTVK
YAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMDDINSEFGQADVVLVLGANDVVNPAAKND
PKSPIAGMPILEAFKAKTIIVNKRSMASGYAGLDNELFYLDKTMMVFGDAKKVIEDMVKA
VENA