Protein Info for Psyr_4862 in Pseudomonas syringae pv. syringae B728a

Annotation: Binding-protein-dependent transport systems inner membrane component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 88 to 112 (25 residues), see Phobius details amino acids 123 to 144 (22 residues), see Phobius details amino acids 162 to 162 (1 residues), see Phobius details amino acids 167 to 167 (1 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 229 to 250 (22 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 105 to 292 (188 residues), 52.4 bits, see alignment E=2.8e-18

Best Hits

Swiss-Prot: 62% identical to POTH_ECOLI: Putrescine transport system permease protein PotH (potH) from Escherichia coli (strain K12)

KEGG orthology group: K11075, putrescine transport system permease protein (inferred from 100% identity to psp:PSPPH_4892)

MetaCyc: 62% identical to putrescine ABC transporter membrane subunit PotH (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotH (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLT3 at UniProt or InterPro

Protein Sequence (306 amino acids)

>Psyr_4862 Binding-protein-dependent transport systems inner membrane component (Pseudomonas syringae pv. syringae B728a)
MKIRKIKRAFERIKPNGRQMVIGVPFLWLFLFFALPFLIVLKISFAEADVAIPPYTEIFS
YADEKLQMVLNFANYTLLSGDDLYVSAYLGSLKMAFISTLLCLLIGYPMAYAIASARKDL
QTVLLLLIMMPTWTAILIRVYAWMGILSNNGLLNAFLMWLGLIDAPLQILNTNLAVYIGV
VYSYLPFMILPLYANLVKHDTSLLEAASDLGSSTFNSFWKITVPLSKNGVIAGCMLVFIP
VVGEFVIPELLGGPETLMIGRVLWQEFFNNRDWPVASALAVIMLLVLIVPIILFNRSQAK
ELEGKA