Protein Info for Psyr_4858 in Pseudomonas syringae pv. syringae B728a

Annotation: Penicillin amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01804: Penicil_amidase" amino acids 42 to 782 (741 residues), 723.7 bits, see alignment E=1.8e-221

Best Hits

KEGG orthology group: K01434, penicillin amidase [EC: 3.5.1.11] (inferred from 100% identity to psb:Psyr_4858)

Predicted SEED Role

"Penicillin acylase II (EC 3.5.1.11)" (EC 3.5.1.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.11

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLT7 at UniProt or InterPro

Protein Sequence (795 amino acids)

>Psyr_4858 Penicillin amidase (Pseudomonas syringae pv. syringae B728a)
MKRSLFILAIIIALLAGGAGWYVNSKLPVRDGEIAMSRLQAPVTLRYDERGVPHIRAENE
ADLYRALGYAHAQDRLFQMEMLRRLARGELAEVLGPNLLETDKLFRSLRIREHADAYVAR
QDKNTPTWKALEAYIDGINQYQDTHARPMEFDALGIPRRRFTPEDTVSIAGYLAYSFAAA
FRTEPLLTYVRDQLGPEYLKVFDLDWHPDGMLDSKPALADADWKGLNQLAQLSHKALEDA
GLPQFEGSNAWVISGSRTQSGKPILAGDPHIRFSVPSVWYEAQLSAPGFELYGHFPALNP
FALLGHNMDFGWSLTMFQNDDVDLIAEKTNPDNPNQVWYHGQWVDMKRTEQQIAVKGQAP
VTLTLLESPHGPIVNNVMGKNAGQAPIAMWWSFLVSANPVLDGFYEANRADTLDKMRSAA
EKIQSPGLNIVWANAKGDIGWWAAAQLPIRPQGVNPTFILDGSTAQADKLGFYPFSANPH
EENPARGYIVSANFQPLSPTGMQIPGYYNPAERGQELNRQLSDASVKWDLAASKALQLDT
QTDYAPSILRPLIPVLRSVVSDPDELALVERLASWKGDYPVDSVGATLFNQFLFDLTEET
FHDELGDAMFETLLSSRVMDAALPRLAADADSPWWNNRNSPHEESRANTVKVAWRASVSH
LRSLYGTNPDEWVWGKAHTLTQGHPLGSQKPLDMIFNVGPYAAPGTHEVPNNLSSSIRPA
PWPVGYGPSTRRLIDFADPAHSLGINPVGQSGVPFDKHYSDQAKAFVNGEYLPQRFSEKD
VAEHTEGVLRLVPGA