Protein Info for Psyr_4842 in Pseudomonas syringae pv. syringae B728a

Annotation: Phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 PF13185: GAF_2" amino acids 16 to 154 (139 residues), 54.1 bits, see alignment E=6.2e-18 PF01590: GAF" amino acids 17 to 153 (137 residues), 64.6 bits, see alignment E=4.3e-21 PF13492: GAF_3" amino acids 18 to 155 (138 residues), 45.2 bits, see alignment E=3.6e-15 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 179 to 732 (554 residues), 446.7 bits, see alignment E=5.5e-138 PF05524: PEP-utilisers_N" amino acids 180 to 301 (122 residues), 96.4 bits, see alignment E=4.2e-31 PF00391: PEP-utilizers" amino acids 327 to 399 (73 residues), 61.7 bits, see alignment E=1.3e-20 PF02896: PEP-utilizers_C" amino acids 427 to 714 (288 residues), 342.5 bits, see alignment E=5.7e-106

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 100% identity to psb:Psyr_4842)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLV3 at UniProt or InterPro

Protein Sequence (759 amino acids)

>Psyr_4842 Phosphoenolpyruvate-protein phosphotransferase (Pseudomonas syringae pv. syringae B728a)
MLNTLRKIVQEVNSAKDLKAALGIIVLRVKEAMGSQVCSVYLLDAEVNRFVLMASEGLNK
RSIGKVSMAPNEGLVGLVGTREEPLNLENAADHPRYRYFAETGEERYASFLGAPIIHHRR
VVGVLVIQQKERRQFDEGEEAFLVTMSAQLAGVIAHAEATGSIRGLGRQGKGIQEAKFVG
VAGSPGAAVGVAVVMLPPADLEVVPDKTVTDIAAELALFQNALEGVRGDMRTLSAKLATQ
LRPEERALFDVYLMMLDDASLGSEVTDVIKTGQWAQGALRSVVSEHVKRFELMDDAYLRE
RASDVKDLGRRLLAYLQEARQQALVYPDNTILVSEELTPAMLGEVPEGKLVGLVSVQGSG
NSHVAILARAMGIPTVMGLVDFPYSKVDGIDLVVDGYHGEVFTNPSEIMRKQFGKVVEEE
RQLSQGLDALRELPCVTLDGHRMPLWVNTGLLADVARAQQRGAEGVGLYRTEVPFMINQR
FPSEKEQLAIYREQLAAFHPLPVTMRSLDIGGDKSLSYFPIKEDNPFLGWRGIRVTLDHP
EIFLVQTRAMLKASEGLNNLRILLPMISSTHEVEEALHLIHRAWGEVRDEGTDVPMPPVG
VMIEVPAAVYQTRDLARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYDYLHPAVLQALQS
VVRDAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWMLRQINLSKAKE
LLAQLMTNDNPQVISSSLQLALKNLGLSRMINPSSVKGH