Protein Info for Psyr_4837 in Pseudomonas syringae pv. syringae B728a

Annotation: Heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase:Heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 transmembrane" amino acids 139 to 158 (20 residues), see Phobius details amino acids 164 to 182 (19 residues), see Phobius details amino acids 194 to 210 (17 residues), see Phobius details amino acids 216 to 234 (19 residues), see Phobius details amino acids 367 to 388 (22 residues), see Phobius details amino acids 395 to 424 (30 residues), see Phobius details amino acids 703 to 722 (20 residues), see Phobius details amino acids 728 to 750 (23 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 179 to 749 (571 residues), 413.8 bits, see alignment E=2.7e-127 TIGR01525: heavy metal translocating P-type ATPase" amino acids 196 to 749 (554 residues), 592.1 bits, see alignment E=2.7e-181 TIGR01494: HAD ATPase, P-type, family IC" amino acids 218 to 731 (514 residues), 278.3 bits, see alignment E=1.8e-86 PF00122: E1-E2_ATPase" amino acids 247 to 428 (182 residues), 194.9 bits, see alignment E=1.4e-61 PF00702: Hydrolase" amino acids 447 to 659 (213 residues), 126.6 bits, see alignment E=2.4e-40

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 100% identity to psb:Psyr_4837)

MetaCyc: 74% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLV8 at UniProt or InterPro

Protein Sequence (752 amino acids)

>Psyr_4837 Heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase:Heavy metal translocating P-type ATPase (Pseudomonas syringae pv. syringae B728a)
MGTTIKNSPGHDHDHDHDHGEHAHEQKPVEHAHSCCSSEAQPAVVTFGEAPAAGGRLSSF
RIEAMDCPTEQTLIQNKLGKLAGVQKLEFNLINRMLGVWHDLPSTDPIREAISSLGMQAE
PVEEGAASAEPAPVVKKHWWPLALSGVAALGAEVVHFASLGPTWVVALLALVSIFSCGLT
TYKKGWIALKNFNLNINALMSIAVTGAILIGQWPEAAMVMFLFTIAELIEAKSLDRARNA
ISGLMQLTPELATVKQADGSWQEVEAKNVELEAIVRIKPGERVGLDGEVVSGSSTIDQAS
ITGESLPVEKTVGDKVFAGTINQAGSLEYRVTAAANNSTLARIIHAVEAAQGSRAPTQRF
VDSFSRIYTPVVFVVALALALVAPLFFGGEWYDWIYRALVLLVVACPCALVISTPVTIVS
GLAAAARKGILIKGGVYLEMGEKLDYLALDKTGTLTHGKPVQTDYVPLNPAVADSAPAIA
ASLAGRSDHPVSQAIAKAADGSLTLHEVSAFEALGGRGVKGEINGQMYHLGNHRLVEELG
LCSPELEARLDALEMQGKTVVLLLDASGPIALFAVADTVKETSREAIAQLHELGIKTVML
TGDNPHTAKAIADQVGIDEAQGNLLPADKLSAIEALYAGNHRVGMVGDGINDAPALARAE
IGFAMAAAGTDTAIETADVALMDDDLRKIPTFIKLSRRTSAVLKQNIVLAIVTKVLFIGI
TFSGLATMWMAVFADMGVSLLVVFNGLRLLKK