Protein Info for Psyr_4828 in Pseudomonas syringae pv. syringae B728a

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR01228: urocanate hydratase" amino acids 20 to 562 (543 residues), 1020 bits, see alignment E=0 PF17391: Urocanase_N" amino acids 20 to 146 (127 residues), 208.1 bits, see alignment E=4.7e-66 PF01175: Urocanase" amino acids 149 to 357 (209 residues), 298.1 bits, see alignment E=5.2e-93 PF17392: Urocanase_C" amino acids 360 to 554 (195 residues), 310.9 bits, see alignment E=4.6e-97

Best Hits

Swiss-Prot: 100% identical to HUTU_PSEU2: Urocanate hydratase (hutU) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to psb:Psyr_4828)

MetaCyc: 89% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLW7 at UniProt or InterPro

Protein Sequence (565 amino acids)

>Psyr_4828 urocanate hydratase (Pseudomonas syringae pv. syringae B728a)
MTENNQHLKQDWTRHREGVVRAARGTQLTAKSWLTEAPLRMLMNNLDPEVAENPNELVVY
GGIGRAARNWECYDKIVESLTQLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPHW
ASWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYDGNLKGRWVLT
AGLGGMGGAQPLAATLAGACSLNIECQQSRIDFRIKTRYVDEQAADLDDALARIAKYTAE
GKAISIALCGNAAEILPEMVRRGVRPDMVTDQTSAHDPLNGYLPKGWTWDEYRARSVSEP
ASVVKAAKQSMAEHVEAMLAFQQAGIPTFDYGNNIRQMAKEVGVTNAFDFPGFVPAYIRP
LFCRGIGPFRWAALSGDPQDIYKTDAKVKELIPDDEHLHNWLDMARERISFQGLPARICW
VGLGQRARLGLAFNEMVRSGELSAPVVIGRDHLDSGSVASPNRETESMRDGSDAVSDWPL
LNALLNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPATGVMR
HADAGYDIAIDCAREQGLNLPMIGR