Protein Info for Psyr_4827 in Pseudomonas syringae pv. syringae B728a

Annotation: BCCT transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 57 to 79 (23 residues), see Phobius details amino acids 94 to 113 (20 residues), see Phobius details amino acids 147 to 167 (21 residues), see Phobius details amino acids 191 to 213 (23 residues), see Phobius details amino acids 233 to 254 (22 residues), see Phobius details amino acids 264 to 283 (20 residues), see Phobius details amino acids 322 to 339 (18 residues), see Phobius details amino acids 351 to 371 (21 residues), see Phobius details amino acids 411 to 434 (24 residues), see Phobius details amino acids 452 to 473 (22 residues), see Phobius details amino acids 479 to 499 (21 residues), see Phobius details PF02028: BCCT" amino acids 18 to 502 (485 residues), 598.4 bits, see alignment E=4.5e-184 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 56 to 502 (447 residues), 512.3 bits, see alignment E=5.5e-158

Best Hits

Swiss-Prot: 46% identical to BETT_ECOLI: High-affinity choline transport protein (betT) from Escherichia coli (strain K12)

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 100% identity to psb:Psyr_4827)

MetaCyc: 46% identical to choline:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-99

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLW8 at UniProt or InterPro

Protein Sequence (664 amino acids)

>Psyr_4827 BCCT transporter (Pseudomonas syringae pv. syringae B728a)
MSSASSSSSGMVRMNAPVFYFAASFILIFGIVVIAFPQASGEWLLAAQNWAANTVGWYYM
MVMTLYLVFVVVTALSGFGKIKLGADHDEPEFSYLSWAGMLFAAGISITLFFFCVSEPLT
HLLQPPQGEGGTAEAARQGMQLLFLHWGLHGWGVFAFVGMALAYFAYRHNLPLALRSALY
PLIGKRINGPIGYAVDGFGIIATIFGLGADMGFGVLHLNSGLDYLFGVAHTHWIQVGLIT
LMMGAAILVAIAGVDKGVRVMSDINMLLACALLLFVLFAGPTQHLLNTLVQNIGDYLGAL
PSKSFDVYAYNEPSDWLGGWTVFYWAWWIAWAPFVGLFIARISRGRTIREFVFGVLLIPL
GFTLAWMSIFGNSAIDQVLNHGMTALGQSAIDDPSMTLYLLLETYPWSKTVIAVTVFISF
VFFVTSADSGTVVLSTLSAKGGNPDEDGPKWLRVFWGVATALITSGLLFSGSIDALKSAV
VLTSLPFSLILLLMMWGLHKAFVMESQRKIAQLYSLAPVSGSRRGGWRQRLSQAVHYPSR
DEVYRFLDQTVRPAIEEVTAVFVEKGLNVVNVPDPSNDSVTLEIGHGEERPFIYQVQMKG
FFTPSFARGGMGSKQLNNRRYYRAEVHLSEGSQDYDLVGYTKEQVINDVLDQYERHMQFL
HLVR