Protein Info for Psyr_4801 in Pseudomonas syringae pv. syringae B728a

Annotation: Outer membrane efflux protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF02321: OEP" amino acids 27 to 200 (174 residues), 68.5 bits, see alignment E=3.4e-23 amino acids 227 to 396 (170 residues), 92.5 bits, see alignment E=1.5e-30

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_4801)

MetaCyc: 67% identical to cobalt-zinc-cadmium resistance protein (Pseudomonas putida KT2440)
RXN1G01-61; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLZ4 at UniProt or InterPro

Protein Sequence (408 amino acids)

>Psyr_4801 Outer membrane efflux protein (Pseudomonas syringae pv. syringae B728a)
MRVGALCLMLLPFIAPGMAMAQGISLPQALEAAFARNPELAAAQWEIGVAEGDRQQAGLI
PNPTVSWEVEDTRRETSTTTVMLSQALELGGKRGARIEAASKGQDAARLELERRGNELRA
EVVQAFYAAARAQAGLELARQSRTLAERGLEVAEGRVRAGKVSPVEATRAQVQLAETDLL
VRRAETLKINSNRELARTTGSPLASFERLDYSDLSPGKLPPSAKLLTALNQSAELRLAQT
QIEQREAALGSERAKRIPDLTVSVGSQYSREERERVNVVGLSMPLPLFDRNQGNVLAASR
RADQSRDLRNAVELKLRTQTQSALDQWTTAAQEVESFDRVILPAAQRAVDTATRGFEMGK
FGFLEVLDAQRTLISARNQYLESLATATEARVAIERIHGDLNRFSLNP