Protein Info for Psyr_4799 in Pseudomonas syringae pv. syringae B728a

Annotation: Heavy metal sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 161 to 187 (27 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 5 to 460 (456 residues), 427.2 bits, see alignment E=4.5e-132 PF00672: HAMP" amino acids 185 to 236 (52 residues), 39.1 bits, see alignment 1.5e-13 PF00512: HisKA" amino acids 243 to 305 (63 residues), 50.1 bits, see alignment E=4.5e-17 PF02518: HATPase_c" amino acids 351 to 461 (111 residues), 101.8 bits, see alignment E=6.3e-33 PF14501: HATPase_c_5" amino acids 356 to 454 (99 residues), 22.5 bits, see alignment E=1.8e-08

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to psb:Psyr_4799)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLZ6 at UniProt or InterPro

Protein Sequence (463 amino acids)

>Psyr_4799 Heavy metal sensor kinase (Pseudomonas syringae pv. syringae B728a)
MKLTRLSTRLGLTVAALIGCLVLVMEMLAYTAISRQLDIRAEDALSEKFRQIEHSMSEGF
LGVADIVQYPHTLRDQIVGHDSFSLTILDSGSPRQELMSVGNVEGRNLPVPDADTLPQGF
QELKSAQGHKILLGYRTVRLKTGQDVLVRLSMDRESDTVLLHAYVTSTCMALPVILLLVG
FGVWWVVRRGLRPLGAFRKVTALISARDLSHRMKVKGLPDELRDLAHAVNFMLHRLDGDV
QQLAQFSDDLAHELRSPMNNLMGRAQVTLSRPRPAEEYKQALESCTEELERMSRMISQML
FLASVSQPAAPLPVEVIDLREEADKVAELFSSSAEDRDITLQVQGHARATGDRLMIQRAI
SNLLSNAIRHGLSGSVITVTLATHEDEVLLAVRNAGDGIDAEHLPRLFDRFYRVHVSRAR
QQGGTGLGLAIVRSIMSLHEGQVTVRSEPGQFTTFSLIFPKLV