Protein Info for Psyr_4668 in Pseudomonas syringae pv. syringae B728a
Annotation: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to YPQQ_PSEPH: Hydrolase in pqqF 5'region from Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)
KEGG orthology group: None (inferred from 100% identity to psb:Psyr_4668)MetaCyc: 66% identical to 5-aminopentanamidase (Pseudomonas putida)
5-aminopentanamidase. [EC: 3.5.1.30]
Predicted SEED Role
"5-aminopentanamidase (EC 3.5.1.30) / Aliphatic amidase AmiE (EC 3.5.1.4)" in subsystem Lysine degradation (EC 3.5.1.30, EC 3.5.1.4)
MetaCyc Pathways
- L-lysine degradation IV (5/5 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- superpathway of acrylonitrile degradation (2/3 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (1/3 steps found)
- indole-3-acetate biosynthesis II (4/12 steps found)
- superpathway of L-lysine degradation (17/43 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Lysine degradation
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.4
Use Curated BLAST to search for 3.5.1.30 or 3.5.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZMC7 at UniProt or InterPro
Protein Sequence (264 amino acids)
>Psyr_4668 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (Pseudomonas syringae pv. syringae B728a) MRVALYQCPPLPLDVSGNLTRLEQQAQAAAKQGAQVLICPEMFLSGYNIGAQAVGELAQA QDGPAATRIAAIAQASGIAILYGYPERAGDGQIYNAVQLIDSQGTRLCNYRKTHLFSELD KSMFVAGDDHYPVVELNGWRLGLLICYDVEFPENTRRLALAGAELILVPTANMLPYDFVC DVTVRARAFENHCYVVYANYCGSEGAIRYCGLSSICAPDGSRPLLAGQDEALLVGTLDKS LLAQARTANDYFVDRRPALYTSLT