Protein Info for Psyr_4634 in Pseudomonas syringae pv. syringae B728a

Annotation: Polynucleotide adenylyltransferase:Metal-dependent phosphohydrolase, HD subdomain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 PF01743: PolyA_pol" amino acids 4 to 122 (119 residues), 65.5 bits, see alignment E=9.5e-22 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 46.2 bits, see alignment E=4.7e-16 PF01966: HD" amino acids 231 to 318 (88 residues), 34.5 bits, see alignment E=3.3e-12

Best Hits

Swiss-Prot: 100% identical to CCA_PSEU2: Multifunctional CCA protein (cca) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 100% identity to psb:Psyr_4634)

MetaCyc: 57% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZMF8 at UniProt or InterPro

Protein Sequence (408 amino acids)

>Psyr_4634 Polynucleotide adenylyltransferase:Metal-dependent phosphohydrolase, HD subdomain protein (Pseudomonas syringae pv. syringae B728a)
MQIYKVGGAVRDRLLGQPVTDIDWVVVGASTEDMLIKGYRPVGTDFPVFLHPLTNEEYAL
ARTERKSGVGYGGFVFHASPEVTLEQDLIRRDLTINAMAEDKDGNLTDPYNGQKDLQARI
LRHVSPAFAEDPLRVLRVARFAARYAGYGFTIAPETLGLMRQLSASGELKALTAERSWKE
ISRALMEEQPQVFIQVLHDCGALKELMPEVEALFGVPQPAAHHPEIDTGVHVLSVLEQSA
RHKHPLTVRWACLLHDLGKGLTPEAEWPRHIAHEHTGLRLIKAVNERFRVPRDCQELALL
VGQYHTHGHRALELKPSTLLELLQSFDVYRRPQRFEEFIAACEMDARGRHGFEQRSYPQA
DYLRGAAEAARAVSVQPLLEQGLKGKELGDALKNERLKALKTYKTSTV