Protein Info for Psyr_4487 in Pseudomonas syringae pv. syringae B728a

Annotation: Carbohydrate kinase, PfkB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF00294: PfkB" amino acids 1 to 295 (295 residues), 224.9 bits, see alignment E=1.5e-70 TIGR02152: ribokinase" amino acids 4 to 297 (294 residues), 339.6 bits, see alignment E=8.5e-106 PF08543: Phos_pyr_kin" amino acids 171 to 280 (110 residues), 36.7 bits, see alignment E=3.1e-13

Best Hits

Swiss-Prot: 42% identical to RBSK_SCHPO: Ribokinase (rbk1) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K00852, ribokinase [EC: 2.7.1.15] (inferred from 100% identity to psb:Psyr_4487)

MetaCyc: 60% identical to deoxyribokinase monomer (Salmonella enterica enterica serovar Typhi)
RXN-14223 [EC: 2.7.1.229]

Predicted SEED Role

"Ribokinase (EC 2.7.1.15)" in subsystem D-ribose utilization or Deoxyribose and Deoxynucleoside Catabolism (EC 2.7.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.15

Use Curated BLAST to search for 2.7.1.15 or 2.7.1.229

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZMV5 at UniProt or InterPro

Protein Sequence (308 amino acids)

>Psyr_4487 Carbohydrate kinase, PfkB (Pseudomonas syringae pv. syringae B728a)
MSKIAVIGSNMVDLITYIERMPAQGETLEAPRFAMGCGGKGANQAAAAALLGADVLMLSK
VGDDAFADTTLANFQRLGIDTRFVERVPGVSSGVAPIFVQENSHNSILIVKGANAHLSPA
DIDRAEAQLRDCALIVLQLEVELATVYHAIAFAHRHAIPVLLNPAPALAGLSREHLAQLD
FLIPNESELALISGQPVTCAEEAAQAARQLVALGVRHVIVTLGEQGALYVGAEGEWQVPG
VKVQARDTTGAGDAFIGCFARHWSQDGNIRQAMHQAVAYSACSVTGLGTQSSYPDASTFA
RFLDASAN