Protein Info for Psyr_4440 in Pseudomonas syringae pv. syringae B728a

Annotation: Heat shock protein DnaJ, N-terminal:Chaperone DnaJ, C-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 PF00226: DnaJ" amino acids 5 to 66 (62 residues), 86.1 bits, see alignment E=1.4e-28 PF01556: DnaJ_C" amino acids 129 to 286 (158 residues), 151 bits, see alignment E=2.7e-48

Best Hits

Swiss-Prot: 96% identical to CBPA_PSESM: Curved DNA-binding protein (cbpA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K05516, curved DNA-binding protein (inferred from 100% identity to psb:Psyr_4440)

Predicted SEED Role

"DnaJ-class molecular chaperone CbpA" in subsystem Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZN02 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Psyr_4440 Heat shock protein DnaJ, N-terminal:Chaperone DnaJ, C-terminal (Pseudomonas syringae pv. syringae B728a)
MDFKDYYKILAVEPTADDKAIKTAYRKLARKYHPDVSKEAGAEDKFKEASEAYEVLSSPE
KRAEYDELRKYGRQGRPFQTPPGWQSRAGAGAGGFEETADFSEFFSSIFGGRPQQGGRTR
NPGRTGQDVEMELAVFLEETLSGESKQVSFKVPQHSPNGQRMSDITKTLNVKIPAGVVDG
ERIRLKGQGAPGVGGGANGDLYLIIRLAPHPLFEVEGHDLVITVPLAPWEAVLGTKVAVP
TLTSRINLTIRPDSQNGQRLRIKGNGLMNKSGERGDLYAQLKIVVPKHTDEATRALWQKL
ADSSSFDPRAQWKG