Protein Info for Psyr_4391 in Pseudomonas syringae pv. syringae B728a

Annotation: type II and III secretion system protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF13629: T2SS-T3SS_pil_N" amino acids 42 to 109 (68 residues), 64.1 bits, see alignment E=8e-22 PF00263: Secretin" amino acids 212 to 373 (162 residues), 145.2 bits, see alignment E=1.5e-46

Best Hits

KEGG orthology group: K02280, pilus assembly protein CpaC (inferred from 100% identity to psb:Psyr_4391)

Predicted SEED Role

"Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZN51 at UniProt or InterPro

Protein Sequence (416 amino acids)

>Psyr_4391 type II and III secretion system protein (Pseudomonas syringae pv. syringae B728a)
MSCCSVPVIKPRLLALVIATLCAPGAWAASGSCAGLAAMPAAVEIDQGIQQEVRSAVPIT
RIAVGDPKIADVHVNGTDGFLLTGVAQGTTSLMVWTACASAPRQSMVFVRGRATTAMVES
VTAPSADAQLPSQVQTDIRFIEVSRTKLKEASTSIFGKGSSNFLFGAPGTVPGVNVTPGA
VGGITPSIPLNNSNFNIVWGGGSSKVLGMINAMENSGYAYTLARPSLVALNGQSASFLAG
GEFPVPVPNGEGNGISIEYKEFGVRLTLTPTVVGRDRILLKVAPEVSELDFSAGITIAGT
TVPALNIRRTDTSIALADGESFVVSGLISSSNSGSVDKFPGLGDIPILGAFFRSSQIQRD
ERELLMIVTPHLVQPLAANARLPSLPGEALRTYDPSFPRLYFLENGDFDRRDGLSK