Protein Info for Psyr_4358 in Pseudomonas syringae pv. syringae B728a

Annotation: Lipoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00510: lipoyl synthase" amino acids 47 to 327 (281 residues), 424.5 bits, see alignment E=1.1e-131 PF16881: LIAS_N" amino acids 50 to 86 (37 residues), 31.5 bits, see alignment 2.1e-11 PF04055: Radical_SAM" amino acids 101 to 264 (164 residues), 78 bits, see alignment E=1e-25

Best Hits

Swiss-Prot: 100% identical to LIPA_PSE14: Lipoyl synthase (lipA) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 100% identity to psp:PSPPH_4399)

MetaCyc: 66% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZN84 at UniProt or InterPro

Protein Sequence (335 amino acids)

>Psyr_4358 Lipoate synthase (Pseudomonas syringae pv. syringae B728a)
MTDTVQTLIPTLDVSERVARPKVEAGVKLRGAEKVARIPVKIIPTVDLPKKPDWIRVRIP
VSPEVDRIKQLLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGR
PKPLDVNEPKSLAIAIADLRLKYVVITSVDRDDLRDGGAQHFADCIREIRLLSPGVQLET
LVPDYRGRMDVALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMVPH
VPTKSGLMLGLGETDEEVIEVMQRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWF
AEEGYKMGFKNVASGPLVRSSYHADEQAKIAKAML